| 货号 | 4135S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W/F |
| 使用方法 | WB(1:2000) F (1:800) |
| 供应商 | CST |
| 背景 | Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13).cdc2蛋白激酶的激活参与调节所有的真核细胞进入有丝分裂。cdc2的激活受多个步骤的调控，包括周期蛋白B的结合、cdc2苏氨酸161位的磷酸化和苏氨酸14位和15位的去磷酸化(1-5)。CDK抑制剂和CDK相关的细胞周期蛋白的水平可通过磷酸化、泛素化和降解来调节，允许了细胞周期事件的化学计量调节(6)。四个周期蛋白B1磷酸化位点(丝氨酸126位，128位，133位和147位)位于胞质滞留信号(CRS)结构域，并可以调节G2/S检验点周期蛋白B1的核迁移(8-10)。周期蛋白B1的磷酸化参与了cdc25C依赖的cdc2 酪氨酸15位的去磷酸化和随后的cdc2/周期蛋白B1激活(11)。尽管cdc2/周期蛋白B1本身能够磷酸化丝氨酸126位和128位(8)，polo样蛋白激酶优先磷酸化丝氨酸133位，也可能磷酸化丝氨酸147位(11-13)。 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from HT29 cells, control (cont.) or nocodazole-treated (50 ng/ml) (M), using Phospho-cyclin B1 (Ser147) Antibody #4131 (upper), Cyclin B1 (V152) Mouse mAb (middle), or Cdc25C Antibody #9522 (lower).western blot方法检测HT29细胞提取物：对照(cont.) 或nocodazole处理(50 ng/ml) (M)。使用的抗体为Phospho-cyclin B1 (Ser147) Antibody #4131 （上图）， Cyclin B1 (V152) Mouse mAb (中图), 或Cdc25C Antibody #9522 (下图). | |
Flow cytometric analysis of untreated Jurkat cells, using Cyclin B1 (V152) Mouse mAb versus propidium iodide (DNA content). The box indicates Cyclin B1-positive cells.流式细胞术分析未处理的Jurkat细胞，使用的抗体为Cyclin B1 (V152) Mouse mAb ，DNA内容物使用碘化丙啶染色。框内所示为Cyclin B1阳性细胞。 |
