| 货号 | 9832S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | GSK-3β (3D10) Mouse mAb recognizes endogenous levels of total GSK-3β protein. This antibody does not cross-react with GSK-3α. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:800) IF-IC (1:250) |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
Confocal immunofluorescent analysis of wild-type mouse embryonic fibroblasts (MEFs) (left) and GSK-3β (-/-) MEFs (right) using GSK-3β (3D10) Mouse mAb (green). Red = Propidium Iodide/RNase (fluorescent DNA dye). (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). 共聚焦免疫荧光分析小鼠胚胎成纤维细胞(MEFs) (左)和GSK-3β (-/-) MEFs (右)，所用抗体为GSK-3β (3D10) Mouse mAb鼠单抗 (绿色)。红色为Propidium Iodide/RNase (荧光 DNA 染料). 。(MEF wild type, GSK-3α (-/-) 细胞由加拿大多伦多大学 Dr. Jim Woodgett友情提供) | |
Flow cytometric analysis of wild-type mouse embryonic fibroblasts (MEFs) (green) and GSK-3β (-/-) MEFs (blue) using GSK-3β (3D10) Mouse mAb. (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). 流式细胞术分析野生型小鼠胚胎成纤维细胞(MEFs) （绿色）和GSK-3β (-/-) MEFs (蓝色)，所用抗体为GSK-3β (3D10) Mouse mAb。(MEF wild type, GSK-3α (-/-) 细胞是加拿大多伦多大学 Dr. Jim Woodgett友情提供) | |
Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) and HeLa cells using GSK-3β (3D10) Mouse mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). western blot 分析wild-type, GSK-3α (-/-), 和GSK3β (-/-) 小鼠胚胎成纤维细胞(MEFs)和Hela细胞，所用抗体为GSK-3β (3D10) Mouse mAb 鼠单抗(upper) 和GSK-3α/β (D75D3) XP® Rabbit mAb 兔单抗#5676 (下). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) 细胞是加拿大多伦多大学 Dr. Jim Woodgett友情提供) |
