| 货号 | 8443S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | hnRNP A1 (D21H11) Rabbit mAb recognizes endogenous levels of total hnRNP A1 protein. This antibody does not cross react with other hnRNP family member proteins. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:100) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10). |
| 存放说明 | -20C |
| 计算分子量 | 34, 40 |
Confocal immunofluorescent analysis of HeLa cells using hnRNP A1 (D21H1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 共聚焦免疫荧光观察hnRNP A1蛋白在HeLa细胞定位，使用hnRNP A1 (D21H1) Rabbit mAb（绿色）标记。DY-554 phalloidin标记微丝蛋白（红色）。 | |
Flow cytometric analysis of HeLa cells using hnRNP A1 (D21H11) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 与Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（红色）比较，使用hnRNP A1 (D21H11) Rabbit mAb (蓝色)流式细胞术分析HeLa细胞。 | |
Western blot analysis of extracts from various cell lines using hnRNP A1 (D21H11) Rabbit mAb. 使用hnRNP A1 (D21H11) Rabbit mAb兔单抗，免疫印迹（Western Blotting）分析不同细胞系提取物。 |
