| 货号 | 8137S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | IDH1 (D2H1) Rabbit mAb recognizes endogenous levels of total IDH1 protein. This antibody does not recognize endogenous IDH2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1). IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3). IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4). IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
Confocal immunofluorescent analysis of 293T cells using IDH1 (D2H1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对293T细胞使用IDH1 (D2H1) Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from various cell lines using IDH1 (D2H1) Rabbit mAb. 对多个细胞系使用IDH1 (D2H1)兔单克隆抗体进行Western blot分析。 | |
Flow cytometric analysis of HeLa cells using IDH1 (D2H1) Rabbit mAb (blue) compared to concentration-matched Rabbit(DA1E) mAb IgG XP® Isotype Control #3900 (red). 使用IDH1 (D2H1) Rabbit mAb兔单抗 (blue)（蓝）和对应的浓度匹配的Rabbit(DA1E) mAb IgG XP? Isotype Control #3900（红），对HeLa细胞进行流式细胞仪分析。 |
