| 货号 | 13838S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:200) F (1:800) |
| 供应商 | CST |
| 背景 | IL-17A is a cysteine-linked, homodimeric, pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (9,10). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNFα, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (9,10). IL-17A is intimately involved in mucosal immunity against bacterial infections (9,11) and has a putative role in some autoimmune disorders (9,12). IL-17A effects appear to be exerted primarily through binding to one of the IL-17 receptor subunits, IL-17RA (13). IL-17 binding induces production of cytokines, chemokines, and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (11,12,14). Phosphorylation of some Jaks and Stats has been observed. |
| 存放说明 | -20C |
| 计算分子量 | 17, 14 |
Western blot analysis of EL4 cells, untreated (-) or treated with TPA #4174 (50 ng/ml), Ionomycin, Calcium Salt #9995 (500 ng/ml), and Brefeldin A #9972 (500 ng/ml; overnight; +), using IL-17A (D1X7L) Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of EL4 cells, untreated (left) or treated with TPA #4174 (50 ng/ml), Ionomycin, Calcium Salt #9995 (500 ng/ml), and Brefeldin A #9972 (500 ng/ml, overnight; right), using IL-17A (D1X7L) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
