| 货号 | 13120S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:1600) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left) or treated with LPS (1 μg/ml, 16 hr; right), using iNOS (D6B6S) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 μg/ml, 8 hr; +), using iNOS (D6B6S) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of Raw309.CR.1 cells, untreated (blue) or treated with LPS (1ug/ml for 16 hours) (green), using iNOS (D6B6S) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 fragment (Alexa Fluor 488 conjugate) #4412 was used as a secondary Ab. |
