| 货号 | 4504S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 目标/特异性 | IRAK1 (D51G7) XP® Rabbit mAb detects endogenous levels of total IRAK1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:800) |
| 供应商 | CST |
| 背景 | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3). |
| 存放说明 | -20C |
| 计算分子量 | 78-105 |
Western blot analysis of extracts from RD, 293 and A20 cells using IRAK1 (D51G7) XP® Rabbit mAb.Western免疫印迹。用IRAK1 (D51G7) XP® Rabbit mAb研究 RD, 293 和 A20细胞的细胞提取液。 | |
Western blot analysis of extracts from COS-7 cells, either mock transfected or transfected with human IRAK1, using IRAK1 (D51G7) XP® Rabbit mAb.Western免疫印迹。用IRAK1 (D51G7) XP® Rabbit mAb研究未转染和转染了人IRAK1的COS-7细胞的细胞提取液。 | |
Western blot analysis of extracts from THP-1, mouse embryonic fibroblast (MEF), RAW264.7 and NIH/3T3 cells using IRAK1 (D51G7) XP® Rabbit mAb.Western免疫印迹。用IRAK1 (D51G7) XP® Rabbit mAb研究 THP-1, 小鼠胚胎纤维原细胞(MEF), RAW264.7 和 NIH/3T3细胞的细胞提取液。 | |
Confocal immunofluorescent analysis of HEK/293 cells using IRAK1 (D51G7) XP® Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析 HEK/293细胞，所用抗体为IRAK1 (D51G7) XP® Rabbit mAb(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® IRAK1 siRNA I (+) or SignalSilence® IRAK1 siRNA II #6228 (+), using IRAK1 (D51G7) XP® Rabbit mAb #4504 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The IRAK1 (D51G7) XP® Rabbit mAb confirms silencing of IRAK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用IRAK1 (D51G7) XP® Rabbit mAb #4504 (上图) 或者 α-Tubulin (11H10) Rabbit mAb #2125 (下图)研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® IRAK1 siRNA I (+) 或 SignalSilence® IRAK1 siRNA II #6228 (+)的HeLa细胞的细胞提取液。IRAK1 (D51G7) XP® Rabbit mAb抗体证实对IRAK1表达的沉默，α-Tubulin (11H10) Rabbit mAb作为上样对照。 | |
Flow cytometric analysis of untreated Raw264.7 cells, +GFP shRNA (blue) or IRAK1 shRNA (green), using IRAK1 (D51G7) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 fragment (Alexa Fluor® 647 conjugate) #4414 was used as a secondary Ab. |
