| 货号 | 4543S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Keratin 17 (D73C7) XP® Rabbit mAb detects endogenous levels of keratin 17 protein. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) XP® Rabbit mAb. As expected, A549 cells are negative for keratin 17.使用Keratin 17 (D73C7) XP® Rabbit mAb，免疫印迹（Western Blot）分析A-431、HeL和A549细胞中Keratin 17蛋白水平。正如所料，keratin 17蛋白在A5491细胞中未被检测。 | |
Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Keratin 17 (D73C7) XP® Rabbit mAb (绿色)标记，共聚焦免疫荧光观察Keratin 17蛋白在HeLa (左图)和A549 (右图)细胞中定位。DY-554 phalloidin标记微丝蛋白（红色）。蓝色=DRAQ5®#4084(DNA荧光染料)。 | |
Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) XP® Rabbit mAb.使用Keratin 17 (D73C7) XP® Rabbit mAb (蓝色)标记，流式细胞仪分析A549细胞(红色)和HeLa细胞(蓝色)。 |
