| 货号 | 2286S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 目标/特异性 | LEF1 (C18A7) Rabbit mAb detects endogenous level of total LEF1 proteins, including the dominant negative forms due to the usage of alternative promoter. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) |
| 供应商 | CST |
| 背景 | LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 25-58 |
Flow cytometric analysis of Jurkat cells, using LEF1 (C18A7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 使用EF1 (C18A7) 兔单抗（蓝色）对Jurkat细胞进行流式细胞分析，使用非特异抗体作为阴性对照（红色）。 | |
Western blot analysis of total cell lysates from mouse thymocytes, DLD1 and HCT15 cells, using LEF1 (C18A7) Rabbit mAb. 使用LEF1（C18A7）兔单抗对小鼠胸腺细胞，DLD1和HCT15细胞全细胞裂解物进行western blot分析。 |
