NF-κB p65 (C22B4) Rabbit mAb【科瑞奥博】

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NF-κB p65 (C22B4) Rabbit mAb

货号： 4764S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	4764S
反应种属	Human/Mouse/Rat/Monkey/Bovine
来源宿主	Rabbit
应用	W/IF-IC/F
目标/特异性	NF-κB p65 (C22B4) Rabbit mAb detects endogenous levels of total NF-κB p65 protein. This antibody may also cross-react with the p50 subunit.
使用方法	WB(1:1000) F (1:400) IF-IC (1:100)

性能
供应商	CST
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
存放说明	-20C
计算分子量	65

参考图片

Western blot analysis of extracts from HeLa (human), PC12 (rat) and Neuro2A (mouse) cell lines using NF-κB p65 (C22B4) Rabbit mAb.Western免疫印迹。用NF-κB p65 (C22B4) Rabbit mAb研究 HeLa (人源), PC12 (大鼠) 和Neuro2A (小鼠)细胞系的细胞提取液。

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TNF-α-treated (#8902, 20ng/ml for 20 min, right), using NF-κB p65 (C22B4) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).共聚焦免疫荧光分析未经处理 (左图) 或 TNF-α处理(#8902, 20ng/ml ,20 min, 右图)的HeLa细胞。所用抗体为NF-κB p65 (C22B4) Rabbit mAb (绿色)。肌动蛋白微丝用 Alexa Fluor® 555 phalloidin (红色)标记。Blue pseudocolor = DRAQ5™ (DNA 荧光染料)。

Flow cytometric analysis of OVCAR8 cells using NF-κB p65 (C22B4) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究OVCAR8细胞，所用抗体为 NF-κB p65 (C22B4) Rabbit mAb (蓝色) 与非特异性抗体(红色)对比。

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (C22B4) Rabbit mAb (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (上图) 和 NF-κB p65 (C22B4) Rabbit mAb (下图)研究经TPA (#9905, 80 nM ,24h)分化和 1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® NF-κB p65 siRNA II (+), or SignalSilence® NF-κB p65 siRNA I #6261 (+), using NF-κB p65 (C22B4) Rabbit mAb #4764 and α-Tubulin (11H10) Rabbit mAb #2125. NF-κB p65 (C22B4) Rabbit mAb confirms silencing of NF-κB p65 and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of NF-κB p65 siRNA.Western免疫印迹。用NF-κB p65 (C22B4) Rabbit mAb #4764 和 α-Tubulin (11H10) Rabbit mAb #2125抗体研究转染了100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® NF-κB p65 siRNA II (+)或 SignalSilence® NF-κB p65 siRNA I #6261 (+)的HeLa 细胞的细胞提取液。NF-κB p65 (C22B4) Rabbit mAb 证实了对NF-κB p65 表达的沉默和 α-Tubulin (11H10) Rabbit mAb 用作上样量的对照和NF-κB p65 siRNA沉默的特异性。