| 货号 | 14574S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:400) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Nucleolin is a multi-functional protein that is one of the major components of the nucleoli (1). Nucleolin plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus (1-3). While the main function of nucleolin is ribosome biogenesis, it plays an important role in various other nuclear activities. Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest (4-6). In addition, nucleolin has been found in a complex with Rad51 and may participate in DNA repair by homologous recombination (7). Nucleolin binds to the catalytic subunit of the human telomerase reverse transcriptase, hTERT, and is thought to be involved in telomere maintenance (8). Nucleolin also possesses histone chaperone activity and is able to enhance the chromatin remodeling efficiency of SWItch/Sucrose Non Fermentable (SWI/SNF) and ATP-dependent chromatin-assembly factor (ACF), remove histone H2A-H2B dimers from nucleosomes, and facilitate the passage of RNA polymerase through chromatin (9). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Confocal immunofluorescent analysis of HeLa cells using Nucleolin (D4C7O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Nucleolin (D4C7O) Rabbit mAb. | |
Immunoprecipitation of nucleolin from Huh7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Nucleolin (D4C7O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Nucleolin (D4C7O) Rabbit mAb. | |
Flow cytometric analysis of HeLa cells using Nucleolin (D4C7O) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
