| 货号 | 2756S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Numb (C29G11) Rabbit mAb detects endogenous levels of total Numb protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9). |
| 存放说明 | -20C |
| 计算分子量 | 72, 74 |
Western blot analysis of extracts from various cell lines using Numb (C29G11) Rabbit mAb.使用Numb (C29G11)兔单抗对多种细胞提取物进行western blot分析。 | |
Flow cytometric analysis of A-204 cells using Numb (C29G11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).使用Numb (C29G11)兔单抗（蓝色）对A-204细胞进行流式细胞分析，对照使用阴性对照抗体（红色）。 | |
Confocal immunofluorescent analysis of HeLa cells using Numb (C29G11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Numb (C29G11)兔单抗（绿色）对HeLa细胞进行激光共聚焦荧光分析。肌动蛋白丝用Alexa Fluor® 554 鬼笔环肽（红色）标记。蓝色假色= DRAQ5® #4084 （DNA荧光染料）。 |
