| 货号 | 31202S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | W F |
| 目标/特异性 | IL-1β (D6D6T) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total IL-1β protein. |
| 使用方法 | WB(1:1000) F (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. |
| 存放说明 | -20C |
| 计算分子量 | 17, 31 |
| 参考文献 | 1 . Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99. 2 . Burns, K. et al. (1998) J Biol Chem 273, 12203-9. 3 . Cao, Z. et al. (1996) Nature 383, 443-6. 4 . Cao, Z. et al. (1996) Science 271, 1128-31. 5 . Wesche, H. et al. (1997) Immunity 7, 837-47. 6 . Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 7 . Thornberry, N.A. et al. (1992) Nature 356, 768-74. 8 . Cerretti, D.P. et al. (1992) Science 256, 97-100. |
Western blot analysis of recombinant mouse IL-1β (mIL-1β) using IL-1β (D6D6T) Rabbit mAb (Mouse Specific). | |
Western blot analysis of extracts from Raw264.7 cells, untreated (-) or treated with LPS (100 ng/mL, 6 hr; +), using IL-1β (D6D6T) Rabbit mAb (Mouse Specific) (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of Raw264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) (green) using IL-1β (D6L6T) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. |
