| 货号 | 9777S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Bovine |
| 来源宿主 | Rat IgG2a |
| 应用 | W/IP/F |
| 目标/特异性 | Pirin (1E8) Rat mAb detects endogenous levels of Pirin protein. This antibody does not cross-react with other proteins in the cupin superfamily. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) |
| 供应商 | CST |
| 背景 | Pirin is a highly conserved nuclear protein and a member of the cupin superfamily of proteins, all of which contain two conserved β-barrel fold domains (1). Pirin functions as a co-factor for NFI/CTF1 and Bcl-3, implicating it in DNA replication, transcriptional activation and apoptosis (2,3). Both human and bacterial pirins catalyze the di-oxygenation of quercetin, one of a class of widespread naturally occurring flavenoid compounds that have anti-inflammatory and anti-cancer activities (4). Flavenoids exert these beneficial activities by functioning as antioxidants that stabilize cellular free radical molecules and by directly modulating cell signaling pathways involving PI 3-kinase, Akt/PKB, PKC and MAP kinases (5). Quercetin has also been directly implicated in the regulation of NF-κB activity; thus, Pirin may exert its apoptotic functions both by directly regulating Bcl-3/NF-κB activity and by modulating quercetin levels in the cell (6). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Flow cytometric analysis of HeLa cells, using Pirin (1E8) Rat mAb (blue) compared to a nonspecific negative control antibody (red). | |
Western blot analysis of cell lysates from HeLa, NIH/3T3, C6 and COS cells, using Pirin (1E8) Rat mAb. |
