货号 | 59754S |
反应种属 | Human/Mouse/Rat |
来源宿主 | Rabbit IgG |
应用 | W/IF-IC/F |
使用方法 | WB(1:1000) F (1:200) IF-IC (1:100) |
供应商 | CST |
背景 | |
存放说明 | -20C |
计算分子量 | 80 |
Confocal immunofluorescent analysis of SK-N-SH (positive, upper) and SK-MEL-2 (negative, lower) cells untreated (left) or treated with TPA #4174 (200 nM, 30 min; right), using PKCα (D7E6E) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of SH-SY5Y cells (green) and SK-MEL-2 cells (blue) using PKCα (D7E6E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using PKCα (D7E6E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |