| 货号 | 9480S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | RagC (D8H5) Rabbit mAb detects endogenous levels of total RagC protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:400) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | mTORC1 kinase complex is a critical component in regulating cell growth (1,2). Its activity is modulated by energy levels, growth factors and amino acids (3,4). Recent studies found that RagA, RagB, RagC and RagD, the four related GTPases, interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Confocal immunofluorescent analysis of HeLa cells using RagC (D8H5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析Hela细胞，所用抗体为RagC (D8H5) Rabbit mAb兔单抗（绿色），肌动蛋白丝用DY-554 phalloidin (红色)标记。蓝色伪彩为DRAQ5 ® #4084 （荧光DNA 染料）。 | |
Western blot analysis of extracts from various cell lines using RagC (D8H5) Rabbit mAb. Western blot 分析不同细胞系的提取物，所用抗体为RagC (D8H5) Rabbit mAb兔单抗。 | |
Flow cytometric analysis of HeLa cells using RagC (D8H5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞术分析Hela细胞，所用抗体为RagC (D8H5) Rabbit mAb兔单抗 (蓝色) 与非特异性阴性对照抗体对比（红色）。 |
