| 货号 | 3223S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Ret (C31B4) Rabbit mAb detects endogenous levels of total Ret protein. This antibody does not cross-react with other related proteins. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4). Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity (5). |
| 存放说明 | -20C |
| 计算分子量 | 170, 175 |
Western blot analysis of extracts from TT cells using Ret (C31B4) Rabbit mAb.用Ret (C31B4) Rabbit mAb对TT细胞的提取物进行免疫印迹检测。 | |
Flow cytometric analysis of TT cells using Ret (C31B4) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).用Ret (C31B4) Rabbit mAb (蓝色)对TT细胞进行流式细胞术检测，非特异阴性对照抗体（红色）。 | |
Confocal immunofluorescent analysis of MCF7 (left) and HeLa cells (right) using Ret (C31B4) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用Ret (C31B4) Rabbit mAb（绿色）对MCF7 (左) 和 HeLa (右)细胞进行免疫共聚焦检测。肌动蛋白微丝用DY-554 phalloidin (红色)标记。蓝色 = DRAQ5® #4084进行的DNA染色 |
