| 货号 | 12556S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F/ChIP |
| 目标/特异性 | RUNX2 (D1L7F) Rabbit mAb recognizes endogenous levels of total RUNX2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:800) IF-IC (1:1600) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Runt-related transcription factor 2 (RUNX2) is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters (1-3). RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) (4-6). RUNX2 is also abnormally expressed in various human cancers including prostate cancer and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 55-62 |
Confocal immunofluorescent analysis of Saos-2 (left) and LNCaP (right) cells using RUNX2 (D1L7F) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用RUNX2 (D1L7F) Rabbit mAb (绿色)对Saos-2细胞 (左) 和LNCaP 细胞(右)进行共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin(红色)标记。 | |
Flow cytometric analysis of LNCaP (blue) and Saos-2 (green) cells using RUNX2 (D1L7F) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
使用RUNX2 (D1L7F) Rabbit mAb对Saos-2 细胞(蓝色) 和LNCaP 细胞(绿色)进行流式分析。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 偶联) #441用作二抗。 | |
