| 货号 | 12891S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | SIX1 (D4A8K) Rabbit mAb recognizes endogenous levels of total SIX1 protein. It does not cross-react with other SIX family proteins. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:400) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | In contrast to the Drosophila ortholog, the vertebrate SIX1 gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13).不同于果蝇同源基因，脊椎动物的SIX1基因在胚胎眼睛发育中并没有太重要的作用。在脊椎动物中，SIX1是中胚层和神经脊源性谱系和男性生殖系统组织发育所必须的(8-10)。SIX1也被认为能够在出生后的肌肉发育过程中调控成年肌肉祖细胞中MyoD的转录(11)。研究支持SIX1在细胞周期调控中的作用，并且在多种癌组织中SIX1表达都会上调，包括乳腺癌，卵巢癌和肝细胞癌(6,12,13)。 |
| 存放说明 | -20C |
| 计算分子量 | 36 |
Western blot analysis of extracts from various cell lines using SIX1 (D4A8K) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). 使用SIX1 (D4A8K) Rabbit mAb兔单抗(上)和β-Actin (D6A8) Rabbit mAb 兔单抗#8457(下)对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of A-204 (positive, left) and Caco-2 (negative, right) cells, using SIX1 (D4A8K) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). 使用SIX1 (D4A8K) Rabbit mAb兔单抗(绿色)对A-204 (阳性，左) 和 Caco-2 (阴性，右)进行免疫荧光共聚焦分析。肌动蛋白丝使用DyLight™ 554 Phalloidin #13054(红色)进行标记。 | |
