| 货号 | 15108S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1000) F (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Z-VAD (50 μM, 3 hr) and Staurosporine #9953 (1 μM, 3 hr; right), using Smac/Diablo (D5S3R) Rabbit mAb. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Smac/Diablo (D5S3R) Rabbit mAb. | |
Immunoprecipitation of Smac/Diablo from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Smac/Diablo (D5S3R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Smac/Diablo Mouse mAb #2954. | |
Flow cytometric analysis of Jurkat cells using Smac/Diablo (D5S3R) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded prostate carcinoma using Smac/Diablo (D5S3R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded colon carcinoma using Smac/Diablo (D5S3R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded lung carcinoma using Smac/Diablo (D5S3R) Rabbit mAb. |
