| 货号 | 23064S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-F/IF-IC/F/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) F (1:100) IF-F (1:400) IF-IC (1:400) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Embryonic stem cells (ESC) derived from the inner cell mass of the blastocyst are unique in their pluripotent capacity and potential for self-renewal (1). Research studies demonstrate that a set of transcription factors that includes Oct-4, Sox2, and Nanog forms a transcriptional network that maintains cells in a pluripotent state (2,3). Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development (4,5). siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (6). Induced overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram both mouse and human somatic cells to a pluripotent state (7,8). Additional evidence demonstrates that Sox2 is also present in adult multipotent progenitors that give rise to some adult epithelial tissues, including several glands, the glandular stomach, testes, and cervix. Sox2 is thought to regulate target gene expression important for survival and regeneration of these tissues (9). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
Western blot analysis of extracts from various cell lines, using Sox2 (D9B8N) Rabbit mAb. | |
Confocal immunofluorescent analysis of F9 (left), 3T3 (middle left), NCCIT (middle right), or HeLa (right) cells using Sox2 (D9B8N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Confocal immunofluorescent analysis of developing mouse E12 foregut (left), spinal cord and somites (middle), or brain (right) using Sox2 (D9B8N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mES cells and either 10 μl of Sox2 (D9B8N) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Flow cytometric analysis of NIH3T3 cells (blue) and F9 cells (green) using Sox2 (D9B8N) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Flow cytometric analysis of HeLa cells (blue) and NTERA cells (green) using Sox2 (D9B8N) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunoprecipitation of Sox2 from F9 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Sox2 (D9B8N) Rabbit mAb. Western blot analysis was performed using Sox2 (D9B8N) Rabbit mAb. |
