| 货号 | 12640S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F/ChIP |
| 目标/特异性 | Stat3 (D3Z2G) Rabbit mAb recognizes endogenous levels of total Stat3 protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:1000) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79, 86 |
Confocal immunofluorescent analysis of serum-starved HeLa cells, untreated (upper left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30 min; upper right), and serum-starved PC-3 cells, untreated (lower right) or treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30 min; lower right), using Stat3 (D3Z2G) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).共聚焦分析血清饥饿的HeLa细胞，未处理(左上图)或Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30分钟; 右上图)，以及血清饥饿的PC-3细胞，未处理(左下图)或Human Interferon-α1 (hIFN-α1) #8927 (100 ng/ml, 30分钟;右下图)，使用抗体是Stat3 (D3Z2G) Rabbit mAb (绿色) 和 β-Actin (8H10D10) Mouse mAb #3700 (红色)。 | |
Flow cytometric analysis of of PC-3 (blue) and HeLa (green) cells using Stat3 (D3Z2G) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.流式细胞术分析PC-3(蓝色)和HeLa细胞(绿色)，使用抗体是Stat3 (D3Z2G) Rabbit mAb。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412用来作为二抗。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml) for 30 minutes, and either 10 μl of Stat3 (D3Z2G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀法检测4 x 106 HepG2细胞中的交联染色质，细胞通过饥饿过夜或者使用Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 分钟) 或10 μl 的 Stat3α (D1A5) XP® Rabbit mAb或2 μl 的 Normal Rabbit IgG #2729处理。使用试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。丰富的DNA通过使用human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663,和SimpleChIP® Human α Satellite Repeat Primers #4486引物进行Real-Time PCR来量化。每个样品中免疫沉淀得到的DNA总量与投入的核酸总量相当。 | |
Western blot analysis of extracts from various cell lines using Stat3 (D3Z2G) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). PC-3 cells are negative for Stat3.Western blot分析多种细胞系的细胞提取物，使用抗体是Stat3 (D3Z2G) Rabbit mAb (上图), 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 PC-3细胞为Stat3阴性。 | |
Immunoprecipitation of Stat3 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Stat3 (D3Z2G) Rabbit mAb (lane 3). Lane 1 is 10% input.对HeLa细胞提取物中的Stat3进行免疫沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 Stat3 (D3Z2G) Rabbit mAb (泳道3)。泳道1为10%的加入量。 |
