| 货号 | 5605S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | c-Myc (D84C12) Rabbit mAb detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins (use Cell Signaling Technology cat. #2276 or #2278). |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 57-65 |
| 参考文献 | 1 . Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702. 2 . Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7. 3 . Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82. 4 . Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99. |
Western blot analysis of extracts from various cell lines using c-Myc (D84C12) XP® Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为 c-Myc (D84C12) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® c-Myc siRNA I #6341, using c-Myc (D84C12) XP® Rabbit mAb. Western blot分析HeLa细胞提取物，空质粒转染或SignalSilence® c-Myc siRNA I #6341 转染；使用抗体为c-Myc (D84C12) XP® Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) XP® Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red). 共聚焦免疫荧光分析HeLa细胞，空质粒转染（左）或SignalSilence® c-Myc siRNA I #6341 转染（右）；使用抗体为c-Myc (D84C12) XP® Rabbit mAb兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。 |
