| 货号 | 9212S |
| 反应种属 | Human/Mouse/Rat/Monkey/GuineaPig |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:200) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Western blot analysis of extracts from NIH/3T3 cells, untreated or UV-treated for the indicated times, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower).western blot方法检测细胞提取物：未处理和按规定时间紫外照射的NIH/3T3细胞，使用的抗体是phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (上图) 和 p38 MAPK Antibody (下图)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAPK Antibody.免疫组织化学方法检测石蜡包埋的人乳腺癌组织，显示了细胞核、细胞质位点，使用的抗体为p38 MAPK Antibody。 | |
Flow cytometric analysis of Jurkat cells, using p38 MAPK Antibody (blue) compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：Jurkat细胞，使用的抗体是p38 MAPK Antibody（蓝色），红色曲线表示非特异性阴性对照抗体。 | |
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower).western blot方法检测细胞提取物：未处理和茴香霉素处理的C6细胞，未处理和紫外照射的NIH/3T3细胞，使用的抗体是phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (上图) 和 p38 MAPK Antibody (下图)。 | |
Confocal immunofluorescent images of HeLa cells-/+ UV light labeled with #9212 p38 MAPK (green) exhibiting cytoplasmic localization in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).激光共聚焦荧光法检测HeLa细胞，未经紫外照射的细胞用#9212 p38 MAPK标记（绿色），图中显示了细胞质的位置。紫外照射的细胞中显示了细胞核的位置。肌动蛋白丝被 DY-554鬼笔环肽标记，呈红色。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p38 MAPK siRNA I #6564 (+) or SignalSilence® p38 MAPK siRNA II (+), using p38 MAPK Antibody #9212 and α-Tubulin (11H10) Rabbit mAb #2125. The p38 MAPK antibody confirms silencing of p38 MAPK expression while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p38 MAPK siRNA.western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® p38 MAPK siRNA I #6564 (+) 、SignalSilence®p38 MAPK siRNA II (+)的HeLa细胞，使用的抗体是p38 MAPK Antibody #9212 和α-Tubulin (11H10) Rabbit mAb #2125。用 p38 MAPK antibody证实了p38 MAPK的沉默表达，而α-Tubulin (11H10) Rabbit mAb被用于负载对照，以及SAPK/JNK siRNA的特异性对照。 |
