| 货号 | 4108S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | LC3A/B Antibody detects endogenous levels of total LC3A and LC3B proteins. Cross-reactivity may exist with LC3C. Stronger reactivity is observed with the type II form of LC3A/B. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11). |
| 存放说明 | -20C |
| 计算分子量 | 14, 16 |
Western blot analysis of extracts from various cell lines, untreated or treated with chloroquine (50 μM, overnight) using LC3A/B Antibody. Western blot分析不同细胞系的提取物，未处理或 chloroquine (氯喹)处理(50 μM, 过夜)，使用的抗体是 LC3A/B Antibody。 | |
Flow cytometric analysis of Hela cells using LC3A/B Antibody (blue) compared to a nonspecific negative control antibody (red). 流式细胞术分析HeLa细胞，使用的抗体是LC3A/B Antibody（蓝色），及非特异的阴性对照抗体（红色）。 | |
Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 uM, overnight; right) using LC3A/B Antibody (green) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
