| 货号 | 9416S |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Mouse |
| 应用 | W/IP/F/E-P |
| 目标/特异性 | Phospho-Tyrosine Mouse mAb (P-Tyr-102) is a high affinity IgG1 monoclonal antibody. ELISAs using a wide variety of phospho-peptides show that P-Tyr-102 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-102 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-102s fine specificity in terms of the sequence context in which it can recognize phospho-tyrosine seems to differ slightly from that of P-Tyr-100 #9411. P-Tyr-102 does not recognize peptides containing phospho-Ser or phospho-Thr. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:2000) IP (1:50) F (1:400) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. |
| 存放说明 | -20C |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tyrosine Mouse mAb (P-Tyr-102). 对石蜡包埋的人乳腺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)进行免疫组化分析。 | |
Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting. 对1mM过钒酸盐裂解前处理30分钟的Jurkat细胞抽提液进行Western blot分析。蛋白质在印记前在二维电泳分离。 | |
Western blot analysis of extracts from sodium vanadate treated (3 mM for 0.5 hour) NIH/3T3 cells, using Phospho-Tyrosine Mouse mAb (P-Tyr-102). 对3mM钒酸钠处理0.5小时的NIH/3T3细胞抽提液使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)进行Western blot分析。 | |
Phospho-Tyrosine Mouse mAb (P-Tyr-102) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (Y* denotes phosphorylated tyrosine.) Phospho-Tyrosine Mouse mAb (P-Tyr-102) ELISA分析：磷酸化和非磷酸化的信噪比。（Y* 代表磷酸化的酪氨酸） | |
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or pervanadate-treated (green), using Phospho-Tyrosine Mouse mAb (P-Tyr-102) compared with a nonspecific negative control antibody (red). 对NIH/3T3细胞，未处理（蓝）或过钒酸盐处理（绿），使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)和非特异性阴性对照抗体（红色），进行流式细胞仪分析。 | |
Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-102). 对石蜡包埋的NCI-H1650移植物，未处理（左）或λ磷酸酶处理（右），使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)进行免疫组化分析。 |
