| 货号 | 13038T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb recognizes endogenous levels of Akt1 protein only when phosphorylated at Thr308. This antibody also recognizes endogenous levels of Akt2 protein when phosphorylated at Thr309 or Akt3 protein when phosphorylated at Thr305. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:6400) IF-IC (1:1600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Franke, T.F. et al. (1997) Cell 88, 435-7. 2 . Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602. 3 . Franke, T.F. et al. (1995) Cell 81, 727-36. 4 . Alessi, D.R. et al. (1996) EMBO J 15, 6541-51. 5 . Sarbassov, D.D. et al. (2005) Science 307, 1098-101. 6 . Jacinto, E. et al. (2006) Cell 127, 125-37. 7 . Cardone, M.H. et al. (1998) Science 282, 1318-21. 8 . Brunet, A. et al. (1999) Cell 96, 857-68. 9 . Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4. 10 . Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 11 . Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8. 12 . Hajduch, E. et al. (2001) FEBS Lett 492, 199-203. 13 . Cross, D.A. et al. (1995) Nature 378, 785-9. 14 . Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. 15 . Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30. 16 . Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52. 17 . Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. 18 . Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. 19 . Manning, B.D. et al. (2002) Mol Cell 10, 151-62. |
Flow cytometric analysis of serum-starved NIH/3T3 cells, untreated (blue) or treated with Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (100 ng/ml, 15 min; green), using Phospho-Akt (Thr308) (D25E6) XP ®Rabbit mAb. Anti-rabbit IgG (H+L), F(ab) Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody. | |
未处理（蓝色）或人血小板源生长因子AA(hPDGF-AA) #8913 (100 ng/ml, 15 min; 绿色)处理的血清饥饿的NIH/3T3细胞，使用Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb兔单抗进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414被用作二抗。 | |
