Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb

货号： 13820T 基本售价： 1600.0 元规格： 20ul

产品信息

概述
货号	13820T
同种亚型	Rabbit IgG
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb recognizes endogenous levels of Smad1 and Smad5 protein when phosphorylated at Ser463/465. This antibody is also predicted to detect Smad9 (Smad8) when phosphorylated at Ser465/467.
使用方法	WB(1:1000) IP (1:50) F (1:400) IF-IC (1:800)

性能
供应商	CST
灵敏度	Endogenous
背景	Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).
存放说明	-20C
计算分子量	60
参考文献	1 . Hogan, B.L. (1996) Genes Dev 10, 1580-94. 2 . Hoodless, P.A. et al. (1996) Cell 85, 489-500. 3 . Klemm, J.D. et al. (1998) Annu Rev Immunol 16, 569-92. 4 . Kretzschmar, M. et al. (1997) Genes Dev 11, 984-95. 5 . Whitman, M. (1998) Genes Dev 12, 2445-62. 6 . Sapkota, G. et al. (2007) Mol Cell 25, 441-54. 7 . Alarcón, C. et al. (2009) Cell 139, 757-69.

参考图片

Western blot analysis of extracts from Hep G2 or MEF cells, untreated (-) or treated with Human BMP2 #4697 (50 ng/ml, 30 min; +), using Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (upper) and Smad1 (D59D7) XP^® Rabbit mAb #6944 (lower).

使用Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb兔单抗 (上) and Smad1 (D59D7) XP^® Rabbit mAb 兔单抗#6944 (下)，对Human BMP2 #4697 (50 ng/ml, 30 min; +)处理或无处理(-)的Hep G2 或 MEF细胞提取物进行Western blot分析。

Flow cytometric analysis of HT-1080 cells, untreated (blue) or treated with Human BMP2 #4697 (green), using Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

使用Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb兔单抗对Human BMP2 #4697 (绿色)处理或无处理(蓝色)的HT-1080细胞进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 488 偶联) #4412做为二抗。

Confocal immunofluorescent analysis of HT-1080 cells, serum-starved (overnight; left) or serum-starved and treated with Human BMP2 #4697 (50 ng/ml, 30 min; right), using Phospho-Smad1/5 (Ser463/Ser465) (D5B10) Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor^® 647 Conjugate) #8584 (red). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087.

血清饥饿（过夜处理，左）或血清饥饿和Human BMP2 #4697 (50 ng/ml, 30 min; 右)处理的HT-1080细胞，使用Phospho-Smad1/5 (Ser463/Ser465) (D5B10) Rabbit mAb兔单抗 (绿色) 和 β-Actin (13E5) Rabbit mAb 兔单抗(Alexa Fluor^® 647 偶联) #8584 (红色)进行共聚焦免疫荧光分析，蓝色伪彩＝Propidium Iodide (PI)/RNase 染色液 #4087。