| 货号 | 2821T |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Phospho-PLCγ1 (Tyr783) Antibody detects PLCγ1 only when phosphorylated at tyrosine 783. It does not cross-react with phosphorylated PLCγ2 or other PLCs. |
| 使用方法 | WB(1:1000) F (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8). |
| 存放说明 | -20C |
| 计算分子量 | 155 |
| 参考文献 | 1 . Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. 2 . Smrcka, A.V. et al. (1991) Science 251, 804-7. 3 . Taylor, S.J. et al. (1991) Nature 350, 516-8. 4 . Yue, C. et al. (1998) J Biol Chem 273, 18023-7. 5 . Yue, C. et al. (2000) J Biol Chem 275, 30220-5. 6 . Margolis, B. et al. (1989) Cell 57, 1101-7. 7 . Kim, H.K. et al. (1991) Cell 65, 435-41. 8 . Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7. |
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCγ1 (Tyr783) Antibody (upper) or PLCγ1 Antibody #2822 (lower). 对NIH/3T3细胞抽提液，未处理或PDGF刺激适当时间，使用Phospho-PLCγ1 (Tyr783)抗体（上图）或PLCγ1抗体#2822（下图）进行Western blot分析。 | |
Flow cytometric analysis of NIH3T3 cells, untreated (blue) or PDGF treated (green), using Phospho-PLCγ1 (Tyr783) Antibody. 对NIH3T3细胞，未经（蓝色）或经过PDGF处理（绿色），使用Phospho-PLCγ1 (Tyr783)抗体进行流式细胞仪分析。 |
