| 货号 | 12290T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | RCAS1 (D2B6N) XP® Rabbit mAb recognizes endogenous levels of total RCAS1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Receptor binding cancer antigen expressed on SiSo cells (RCAS1) is also known as estrogen receptor-binding fragment-associated gene 9 (EBAG9). Originally identified as an estrogen-inducible gene (1), RCAS1 was recently found to play a novel role in the adaptive immune response by negatively regulating the cytolytic activity of cytotoxic T lymphocytes (CTLs) (2). RCAS1 is conserved in phylogeny and is ubiquitously expressed in most human tissues and cells (3,4). There is evidence that tissue expression of RCAS1 is increased in a variety of malignancies, including cancers of the gastrointestinal tract, liver, lung, breast, ovary, endometrium, and cervix. Research studies have shown that levels of RCAS1 tissue expression are negatively correlated with the prognosis of patients harboring the aforementioned malignancies (4). It is also noteworthy that research studies have detected elevated levels of RCAS1 in the sera of cancer patients (4). Initial studies indicated that RCAS1 was secreted from cancer cells and functioned as a ligand for a putative receptor expressed on NK cells, as well as T and B lymphocytes, inducing their apoptosis, which enabled cancer cells to evade immune surveillance (5,6). Subsequent studies have identified RCAS1 as a type III transmembrane Golgi protein with the ability to regulate vesicle formation, secretion, and protein glycosylation (2,7-9). Indeed, it has been shown that RCAS1 overexpression negatively regulates the cytolytic function of CTLs by negatively regulating protein trafficking from the trans-Golgi to secretory lysosomes (2). Furthermore, RCAS1 overexpression delays vesicle transport from the ER to Golgi and causes components of the ER quality control and glycosylation machinery to mislocalize. As a consequence, RCAS1 induces the deposition of tumor-associated glycan antigens on the cell surface, which are thought to contribute to tumor pathogenesis through the mediation of adhesion, invasion, and metastasis (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
| 参考文献 | 1 . Watanabe, T. et al. (1998) Mol Cell Biol 18, 442-9. 2 . Rüder, C. et al. (2009) J Clin Invest 119, 2184-203. 3 . Tsuchiya, F. et al. (2001) Biochem Biophys Res Commun 284, 2-10. 4 . Giaginis, C. et al. (2009) Histol Histopathol 24, 761-76. 5 . Matsushima, T. et al. (2001) Blood 98, 313-21. 6 . Nakashima, M. et al. (1999) Nat Med 5, 938-42. 7 . Reimer, T.A. et al. (2005) BMC Cancer 5, 47. 8 . Wolf, J. et al. (2010) FASEB J 24, 4000-19. 9 . Engelsberg, A. et al. (2003) J Biol Chem 278, 22998-3007. |
Flow cytometric analysis of Jurkat cells using RCAS1 (D2B6N) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 流式细胞术检测Jurkat细胞活性，使用的抗体为RCAS1 (D2B6N) XP® Rabbit mAb兔单抗（蓝色）与使用浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900#9078（红色）相比较。 | |
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; right), using RCAS1 (D2B6N) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光术检测未处理（左）或经Brefeldin A #9972 (5 μg/ml, 1 hr; 右)处理的MCF7 细胞，使用的抗体为 RCAS1 (D2B6N) XP® Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。蓝色伪彩=DRAQ5® #4084 (荧光 DNA 染料)。 | |
Western blot analysis of extracts from various cell lines using RCAS1 (D2B6N) XP® Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为RCAS1 (D2B6N) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RCAS1 (hRCAS1-Myc/DDK; +), using RCAS1 (D2B6N) XP® Rabbit mAb. Western blot方法检测293T细胞提取物，细胞分别用空白对照（-）或构建的带有Myc/DDK标签的全长人RCAS1 (hRCAS1-Myc/DDK; +)转染，使用的抗体为 RCAS1 (D2B6N) XP® Rabbit mAb兔单抗。 | |
Immunoprecipitation of RCAS1 from HCT 116 extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RCAS1 (D2B6N) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RCAS1 (D2B6N) XP® Rabbit mAb. 免疫沉淀法检测 HCT 116提取物中的RCAS1，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（泳道 2）或 RCAS1 (D2B6N) XP® Rabbit mAb兔单抗(泳道 3)。泳道1为10% input对照。 Western blot 检测中使用的是 RCAS1 (D2B6N) XP® Rabbit mAb兔单抗。 |
