Cox2 (D5H5) XP® Rabbit mAb【科瑞奥博】

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Cox2 (D5H5) XP® Rabbit mAb

货号： 12282T 基本售价： 1550.0 元规格： 20ul

产品信息

概述
货号	12282T
同种亚型	Rabbit IgG
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IHC-P/IF-IC/F
目标/特异性	Cox2 (D5H5) XP® Rabbit mAb recognizes endogenous levels of total Cox2 protein.
使用方法	WB(1:1000) IHC-P (1:600) F (1:200) IF-IC (1:800)

性能
供应商	CST
灵敏度	Endogenous
背景	Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimers disease by potentiating γ-secretase activity and β-amyloid generation (6).
存放说明	-20C
计算分子量	74
参考文献	1 . Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6. 2 . Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120. 3 . ONeill, G.P. et al. (1994) Mol Pharmacol 45, 245-54. 4 . Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97. 5 . Wang, Q. et al. (2001) Infect Immun 69, 2270-6. 6 . Qin, W. et al. (2003) J Biol Chem 278, 50970-7. 7 . Onodera, Y. et al. (2015) FEBS Open Bio 5, 492-501.

参考图片

Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left; -) or LPS-treated (right; 1ug/ml, 24 hrs; +), using Cox2 (D5H5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对RAW264.7进行共聚焦分析，未处理（左图；—）或LPS处理（右图；1ug/ml, 24 小时; +），使用抗体是Cox2 (D5H5) XP® Rabbit mAb (绿色)。 Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料).

Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or LPS-treated (1μg/ml, 24 hrs; green), using Cox2 (D5H5) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.对RAW264.7细胞进行流式细胞分析，未处理(蓝色)或LPS处理 (1μg/ml, 24 小时; 绿色)，使用抗体是Cox2 (D5H5) XP® Rabbit mAb。 Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Cox2 (D5H5) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).对石蜡包埋的人源卵巢癌组织进行免疫组化分析，使用看题是Cox2 (D5H5) XP® Rabbit mAb ，对照肽(左图)或抗原特异性肽(右图)。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Cox2 (D5H5) XP® Rabbit mAb.对石蜡包埋的人源卵巢癌组织进行免疫组化，使用抗体是Cox2 (D5H5) XP® Rabbit mAb。

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 μg/ml, 24 hr; +), using Cox2 (D5H5) XP® Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457.Western blot分析RAW264.7细胞的细胞提取物，未处理(-)或LPS-处理(1 μg/ml, 24 小时; +), 使用抗体是Cox2 (D5H5) XP® Rabbit mAb 或 β-Actin (D6A8) Rabbit mAb #8457。