| 货号 | 12165T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Xenopus/Zebrafish/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | HSP60 (D6F1) XP® Rabbit mAb recognizes endogenous levels of total HSP60 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimers, coronary artery diseases, MS, and diabetes (5-8). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Hartl, F.U. (1996) Nature 381, 571-579. 2 . Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366. 3 . Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858. 4 . Jindal, S. et al. (1989) Mol Cell Biol 9, 2279-83. 5 . Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938. 6 . Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58. 7 . Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128. 8 . Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297. |
Confocal immunofluorescent analysis of A-204 cells using HSP60 (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光分析A-204细胞，使用的抗体为HSP60 (D6F1) XP® Rabbit mAb兔单抗 (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HSP60 (D6F1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化分别检测在对照多肽（左）或抗原特异性多肽（右）存在的情况下石蜡包埋的人卵巢癌，所使用的抗体为 HSP60 (D6F1) XP® Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HSP60 (D6F1) XP® Rabbit mAb. 免疫组化检测石蜡包埋的人乳腺癌，所使用的抗体为 HSP60 (D6F1) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from various cell lines using HSP60 (D6F1) XP® Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为HSP60 (D6F1) XP® Rabbit mAb兔单抗。 | |
Flow cytometric analysis of HeLa cells using HSP60 (D6F1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. 流式细胞术检测HeLa细胞活性，使用的抗体为HSP60 (D6F1) XP® Rabbit mAb兔单抗 （蓝色）与使用浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900的相比较（红色）。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412作为二抗使用。 | |
Immunohistochemical analysis of paraffin-embedded mouse lung using HSP60 (D6F1) XP® Rabbit mAb. 免疫组化检测石蜡包埋的小鼠肺组织，所使用的抗体为 HSP60 (D6F1) XP® Rabbit mAb兔单抗。 |
