| 货号 | 12456T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | GSK-3β (D5C5Z) XP® Rabbit mAb recognizes endogenous levels of total GSK-3β protein. This antibody does not cross-react with GSK-3α protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
| 参考文献 | 1 . Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9. 2 . Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41. 3 . Cross, D.A. et al. (1995) Nature 378, 785-9. 4 . Nusse, R. (1997) Cell 89, 321-3. 5 . Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. |
Confocal immunofluorescent analysis of wild-type mouse embryonic fibroblasts (MEFs) (left), GSK-3α (-/-) MEFs (center)and GSK-3β (-/-) MEFs (right) using GSK-3β (D5C5Z) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type, GSK-3α (-/-), and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). | |
使用GSK-3β (D5C5Z) XP®Rabbit mAb兔单抗（绿色）对野生型小鼠胚胎成纤维细胞（MEFs）（左），GSK-3α (-/-) MEFs（中）和GSK-3β (-/-) MEFs（右）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye). (MEF 野生型, GSK-3β (-/-),和GSK-3α (-/-) 细胞 由加拿大多伦多大学的Dr. Jim Woodgett友情提供). | |
Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) using GSK-3β (D5C5Z) XP® Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-), and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). 使用GSK-3β (D5C5Z) XP®Rabbit mAb兔单抗（上）和GSK-3α/β (D75D3) XP® Rabbit mAb兔单抗 #5676（下）对野生型，GSK-3α (-/-), 和 GSK3β (-/-)小鼠成纤维细胞（MEFs）细胞提取物进行western blot分析。(MEF 野生型, GSK-3β (-/-),和GSK-3α (-/-) 细胞 由加拿大多伦多大学的Dr. Jim Woodgett友情提供). | |
Immunohistochemical analysis of paraffin-embedded MEF cell pellets, wild type (left), GSK-3α (-/-) (middle) and GSK-3β (-/-) (right) using GSK-3β (D5C5Z) XP® Rabbit mAb. (MEF wild type, GSK-3β (-/-), and GSK-3α (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). 使用GSK-3β (D5C5Z) XP®Rabbit mAb兔单抗对野生型（左），GSK-3α (-/-)（中）和GSK-3β(-/-)（右）对石蜡包埋的MEF细胞进行免疫组化分析。(MEF 野生型, GSK-3β (-/-),和GSK-3α (-/-) 细胞 由加拿大多伦多大学的Dr. Jim Woodgett友情提供). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GSK-3β (D5C5Z) XP® Rabbit mAb. 使用GSK-3β (D5C5Z) XP®Rabbit mAb兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunoprecipitation of GSK-3β from PC-12 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or GSK-3β (D5C5Z) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using GSK-3β (D5C5Z) XP® Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或GSK-3β (D5C5Z) XP® Rabbit mAb 兔单抗(lane 3)对PC-12细胞提取物中的GSK-3β进行免疫沉淀实验。Lane 1 是10%上样量。使用GSK-3β (D5C5Z) XP® Rabbit mAb兔单抗进行western blot分析。 |
