| 货号 | 12509T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Keratin 17 (D12E5) XP® Rabbit mAb recognizes endogenous levels of total keratin 17 protein. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Moll, R. et al. (1982) Cell 31, 11-24. 2 . Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13. 3 . Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4. 4 . Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66. 5 . Zatloukal, K. et al. (2004) J Pathol 204, 367-76. 6 . Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85. 7 . Paladini, R.D. et al. (1996) J Cell Biol 132, 381-97. 8 . Kim, S. et al. (2006) Nature 441, 362-5. |
Confocal immunofluorescent analysis of HeLa (positive, left) and HCC827 (negative, right) cells using Keratin 17 (D12E5) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of SK-MEL-28 (blue) and HeLa (green) cells using Keratin 17 (D12E5) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using Keratin 17 (D12E5) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
