| 货号 | 13110T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F/IF-IC/F |
| 目标/特异性 | PCNA (D3H8P) XP® Rabbit mAb recognizes endogenous levels of total PCNA protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:8000) F (1:100) IF-F (1:800) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) mouse mAb has been used to assess PCNA levels in hundreds of scientific studies. |
| 存放说明 | -20C |
| 计算分子量 | 36 |
| 参考文献 | 1 . Kelman, Z. and ODonnell, M. (1995) Nucleic Acids Res 23, 3613-20. 2 . Krishna, T.S. et al. (1994) Cell 79, 1233-43. 3 . Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60. |
Confocal immunofluorescent analysis of HeLa cells using PCNA (D3H8P) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). 使用PCNA (D3H8P) XP® Rabbit mAb（绿色）和β-Actin (8H10D10) Mouse mAb #3700（红色）对HeLa细胞进行激光共聚焦免疫荧光分析。 | |
Confocal immunofluorescent analysis of rat small intestine using PCNA (D3H8P) XP® Rabbit mAb (green) showing staining of proliferative cells in intestinal crypts and β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用PCNA (D3H8P) XP® Rabbit mAb（绿色）和β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677（红色）对大鼠小肠组织进行激光共聚焦免疫荧光分析以显示小肠隐窝处的增殖细胞。蓝色假色= DRAQ5® #4084（fluorescent DNA dye）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PCNA (D3H8P) XP® Rabbit mAb.使用PCNA (D3H8P) XP® Rabbit mAb对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PCNA (D3H8P) XP® Rabbit mAb.使用PCNA (D3H8P) XP® Rabbit mAb对石蜡包埋的人结肠癌组织进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using PCNA (D3H8P) XP® Rabbit mAb. 使用PCNA (D3H8P) XP® Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of PCNA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PCNA (D3H8P) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using PCNA (D3H8P) XP® Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 PCNA (D3H8P) XP® Rabbit mAb (lane 3)对HeLa细胞提取物的PCNA进行免疫沉淀实验。Lane 1是10%上样量。使用PCNA (D3H8P) XP®Rabbit mAb进行western blot。 | |
Flow cytometric analysis of Jurkat cells using PCNA (D3H8P) XP® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. 使用PCNA (D3H8P) XP® Rabbit mAb和碘化丙啶(PI)/RNase Staining Solution #4087以计算DNA量。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412用作二抗。 |
