| 货号 | 13901T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | Vinculin (E1E9V) XP® recognizes endogenous levels of total vinculin protein. This antibody also reacts with metavinculin, a 145 kDa splice variant of vinculin. |
| 使用方法 | WB(1:1000) IHC-P (1:150) F (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4). Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail (1). In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2). Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5,6). This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2,4). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7,8). This is supported by a recently demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9). |
| 存放说明 | -20C |
| 计算分子量 | 124 |
| 参考文献 | 1 . Izard, T. et al. (2004) Nature 427, 171-5. 2 . Humphries, J.D. et al. (2007) J Cell Biol 179, 1043-57. 3 . Witt, S. et al. (2004) J Biol Chem 279, 31533-43. 4 . Xu, W. et al. (1998) Development 125, 327-37. 5 . Ziegler, W.H. et al. (2002) J Biol Chem 277, 7396-404. 6 . Zhang, Z. et al. (2004) Mol Biol Cell 15, 4234-47. 7 . Rodríguez Fernández, J.L. et al. (1993) J Cell Biol 122, 1285-94. 8 . Samuels, M. et al. (1993) J Cell Biol 121, 909-21. 9 . Yang, H.J. et al. (2010) Cancer Invest 28, 127-34. |
Immunohistochemical analysis of paraffin-embedded Hs578T (left) and Jurkat (right) cell pellets using Vinculin (E1E9V) XP(R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using Vinculin (E1E9V) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Vinculin (E1E9V) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb, #6487 (lower). | |
Flow cytometric analysis of Jurkat cells (blue) and HeLa cells (green) using Vinculin (E1E9V) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma in situ using Vinculin (E1E9V) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Vinculin (E1E9V) XP® Rabbit mAb. |
