| 货号 | 2845T |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 目标/特异性 | 4E-BP2 Antibody detects endogenous levels of total 4E-BP2, independent of phosphorylation. This antibody does not cross-react significantly with 4E-BP1. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:50) F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous (F), Transfected (W) |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15 to 20 |
| 参考文献 | 1 . Pause, A. et al. (1994) Nature 371, 762-7. 2 . Brunn, G.J. et al. (1997) Science 277, 99-101. 3 . Gingras, A.C. et al. (1998) Genes Dev 12, 502-13. 4 . Fadden, P. et al. (1997) J Biol Chem 272, 10240-7. 5 . Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37. 6 . Lin, T.A. and Lawrence, J.C. (1996) J. Biol. Chem. 271, 30199-30204. |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic and nuclear localization, using 4E-BP2 Antibody. 使用4E-BP2 Antibody，免疫组化分析人类结肠癌石蜡切片，结果显示出4E-BP2在细胞质和细胞核的定位。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using 4E-BP2 Antibody. 使用4E-BP2 Antibody，免疫组化分析人类肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human follicular carcinoma (thyroid), using 4E-BP2 Antibody. 使用4E-BP2 Antibody，免疫组化分析人类滤胞癌组织石蜡切片。 | |
Western blot analysis of extracts from A673 cells, untreated or nocodazole-treated (100 ng/ml, 16 hrs), using 4E-BP2 Antibody (upper) or 4E-BP1 Antibody #9452 (lower). Extracts were treated with lambda phosphatase NEB#P0753 (10,000 U/ml for 1 hour) to dephosphorylate both proteins. 使用4E-BP2 Antibody (上图)和4E-BP1 Antibody #9452 (下图)，免疫组化（Western blot）分析A673细胞裂解物，细胞分为未处理组和nocodazole处理组(100 ng/ml, 16小时)。使用lambda phosphatase NEB#P0753 (10,000 U/ml，1小时)处理裂解物从而达到去除蛋白的磷酸化。 | |
Flow cytometric analysis of HeLa cells, using 4E-BP2 Antibody (blue) compared to a nonspecifc negative control antibody (red). 与非特异的阴性control antibody（红色）比较，使用4E-BP2 Antibody (蓝色) 流式细胞术分析HeLa细胞。 | |
Western blot analysis of bacterially expressed GST-4E-BP1 and of extracts from NIH/3T3 cells, using 4E-BP2 Antibody and 4E-BP1 Antibody #9452. 使用4E-BP2 Antibody和4E-BP1 Antibody #9452，免疫印迹（Western blot）分析原核表达的GST-4E-BP1以及NIH/3T3细胞的提取物。 |
