| 货号 | 12653T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | FoxP3 (D6O8R) XP® Rabbit mAb recognizes endogenous levels of total FoxP3 protein. This antibody recognizes both human and mouse FoxP3 protein. |
| 使用方法 | WB(1:1000) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59. 2 . Shu, W. et al. (2001) J Biol Chem 276, 27488-97. 3 . Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8. 4 . Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65. 5 . Li, S. et al. (2004) Mol Cell Biol 24, 809-22. 6 . Ochs, H.D. et al. (2007) Immunol Res 38, 112-21. 7 . Bennett, C.L. et al. (2001) Nat Genet 27, 20-1. 8 . Kasprowicz, D.J. et al. (2003) J Immunol 171, 1216-23. 9 . Zuo, T. et al. (2007) Cell 129, 1275-86. 10 . Zuo, T. et al. (2007) J Clin Invest 117, 3765-73. 11 . Wang, L. et al. (2009) Cancer Cell 16, 336-46. |
Flow cytometric analysis of murine spleen lymphocytes using a CD25 antibody and FoxP3 (D6O8R) XP® Rabbit mAb. Anti-rabbit IgG (H+L), (F(ab)2 ) Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on a gated CD4+ T cell population.使用CD25抗体和FoxP3 (D6O8R) XP®兔mAb对小鼠脾脏淋巴细胞进行流式分析。Anti-rabbit IgG (H+L), (F(ab)2 ) Fragment (Alexa Fluor® 488 Conjugate) #4412作为二抗。用CD4+ T细胞群进行分析。 |
