| 货号 | 2056T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | PKCalpha Antibody detects endogenous levels of total PKCα protein. The antibody does not cross-react with endogenous levels of other PKC isoforms. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Nishizuka, Y. (1984) Nature 308, 693-8. 2 . Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403. 3 . Mellor, H. and Parker, P.J. (1998) Biochem J 332 ( Pt 2), 281-92. 4 . Ron, D. and Kazanietz, M.G. (1999) FASEB J 13, 1658-76. 5 . Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep 1, 399-403. 6 . Baron, C.L. and Malhotra, V. (2002) Science 295, 325-8. 7 . Flynn, P. et al. (2000) J Biol Chem 275, 11064-70. |
Western blot analysis of extracts of HeLa, COS, C6 and NIH/3T3 cells, using PKCα Antibody. 对HeLa、COS、C6和NIH/3T3细胞，使用PKCα抗体进行Western blot分析。 | |
Western blot analysis of extracts of Baculovirus expressed PKC isoforms demonstrating the isoform-specificity of PKCα Antibody. 对Baculovirus表达的PKC亚型的Western blot分析显示出PKCα的亚型特异性。 | |
Flow cytometric analysis of untreated 293 cells, using PKCα Antibody (blue) compared to a nonspecific negative control antibody (red). 使用PKCα抗体（蓝）和非特异的阴性对照抗体（红），对293细胞进行流式细胞仪分析。 | |
Confocal immunofluorescent images of C6 cells serum-starved (left) or TPA #9905 treated (center), labeled with PKCα Antibody (green) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 共聚焦显微图像显示C6细胞，血清饥饿（左）或TPA #9905处理（中），以PKCα抗体（绿）标记，并与同类对照组（右）比对。肌动蛋白用Alexa Fluor? 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5™(荧光DNA染料)。 |
