| 货号 | 2128T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | β-Tubulin (9F3) Rabbit mAb detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. |
Confocal immunofluorescent analysis of HeLa cells, using β-Tubulin (9F3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用β-Tubulin (9F3) Rabbit mAb(绿色)标记，共聚焦免疫荧光观察β-Tubulin蛋白在HeLa细胞中定位。蓝色伪彩=DRAQ5®#4084(DNA荧光染料)。 | |
Flow cytometric analysis of NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用β-Tubulin (9F3) Rabbit mAb (蓝色)标记进行流式细胞仪分析NIH/3T3细胞。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Tubulin (9F3) Rabbit mAb. 使用β-Tubulin (9F3) Rabbit mAb兔单抗，免疫组化分析人源肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right). 使用β-Tubulin (9F3) Rabbit mAb，并且提前与control peptide (左图)或β-Tubulin Blocking Peptide #1032 (右图)孵育，免疫组化分析人源乳腺癌石蜡切片。 | |
Western blot analysis of extracts from COS-7, NIH/3T3 and PC12 cells, using β-Tubulin (9F3) Rabbit mAb. 使用β-Tubulin (9F3) Rabbit mAb，免疫印迹（Western Blot）分析COS-7、NIH/3T3和PC12细胞中β-Tubulin蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human melanoma, using β-Tubulin (9F3) Rabbit mAb. 使用β-Tubulin (9F3) Rabbit mAb，免疫组化分析黑素瘤组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human glioblastoma, using β-Tubulin (9F3) Rabbit mAb. 使用β-Tubulin (9F3) Rabbit mAb，免疫组化分析人源恶性胶质瘤组织石蜡切片。 |
