| 货号 | 2230T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | LEF1 (C12A5) Rabbit mAb detects endogenous level of total LEF1 protein. It does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. |
| 使用方法 | WB(1:1000) F (1:400) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 25-58 |
| 参考文献 | 1 . Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52. 2 . Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32. 3 . Brantjes, H. et al. (2002) Biol Chem 383, 255-61. 4 . Reya, T. and Clevers, H. (2005) Nature 434, 843-50. 5 . Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810. 6 . Hovanes, K. et al. (2000) Nucleic Acids Res 28, 1994-2003. 7 . Hovanes, K. et al. (2001) Nat Genet 28, 53-7. 8 . Kobielak, A. et al. (2001) Acta Biochim Pol 48, 221-6. |
Flow cytometric analysis of untreated Jurkat cells using LEF1 (C12A5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 使用LEF1 Rabbit mAb (C12A5)兔单抗（蓝色）对未处理的Jurkat细胞进行流式细胞分析，非特异抗体作为阴性对照（红色）。 | |
Western blot analysis of total cell lysates from HCT15, DLD1 and mouse thymocytes using LEF1 (C12A5) Rabbit mAb. 使用LEF1 (C12A5) Rabbit mAb兔单抗对HCT15, DLD1和小鼠胸腺细胞全细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of HCT-15 cells using LEF1 (C12A5) Rabbit mAb (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red). 使用LEF1 (C12A5) Rabbit mAb 兔单抗(绿色)对HCT-15细胞进行共聚焦免疫荧光分析。肌动蛋白丝使用DyLight™ 554 Phalloidin #13054 (红色)标记。 |
