RAT ANTI MOUSE CD8:Low Endotoxin【科瑞奥博】

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RAT ANTI MOUSE CD8:Low Endotoxin

货号： MCA1108EL 基本售价： 4885.0 元规格： 0.5 mg

产品信息

概述
货号	MCA1108EL
克隆号	YTS105.18
同种亚型	IgG2a
反应种属	Mouse
来源宿主	Rat
应用	C, F, IF

性能
供应商	Bio-Rad Antibodies
运输条件
存放说明	Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20^oC only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Staining of mouse spleen cells with Rat anti Mouse CD8 (MCA1108G)

Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody (MCA74), green in A and Rat anti Mouse CD8 antibody (MCA1108), red in B, Merged image in C

Published customer image:
Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 µg/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.

From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, et al. (2009) Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE 4(12): e8291.

Published customer image:
NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8–positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 µm.

From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.

Published customer image:
The effect of NK cell or CD8+T cell depletion on NKG2D-positive lymphocyte localization in tumors. Groups of mice (n = 3 per treatment group) were subjected to one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12. Tumor sections were stained as detailed in the Figure 3 legend. (A) Detection of NKG2D-positive cells in tumors from NK cell–depleted mice, (B) Absence of NKG2D-positive cells in CD8+T cell–depleted mice. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 µm.

From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.

Published customer image:
The effect of NKG2D-blocking antibody on NKG2D-positive CD8+T cells and CD8+T cell accumulation in tumors. 4T-1 tumor–bearing mice were subjected to one of two treatments: Dox plus IL-12 plus control IgG or Dox plus IL-12 plus NKG2D-blocking antibody (n = 3 per treatment). (A) NKG2D expression in CD8+T cells was determined as described for Figure 1 (n.s., not significant). (B) The presence of NKG2D/CD8–positive cells in tumor sections after the indicated treatments were determined as described for Figure 3.

From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.

Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8α antibody (MCA1108) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) for detection. Low power

Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MCA711), green in A and Rat anti Mouse CD8, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA711), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. High power

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MCA1439), green in A and Rat anti Mouse CD8 (MCA1108), red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power