RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE【科瑞奥博】

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RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE

货号： MCA771PE 基本售价： 3557.0 元规格： 100 TESTS

产品信息

概述
货号	MCA771PE
克隆号	7/4
同种亚型	IgG2a
反应种属	Mouse
来源宿主	Rat
应用	F

性能
供应商	Bio-Rad Antibodies
溶解方法	Pack Size: 100 TestsReconstitute with 1ml distilled waterPack Size: 500 TestsReconstitute with 5.0ml distilled water
运输条件
存放说明	Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 50 µg, 0.1 mg, 25 µgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 Tests, 500 TestsPrior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 Tests/0.25mlStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 50 µg, 0.1 mg, 25 µgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 50 µg, 0.1 mg, 25 µgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 Tests, 500 TestsPrior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 Tests/0.25mlStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 Tests, 500 TestsPrior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 Tests/0.25mlStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 50 µg, 0.1 mg, 25 µgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Flow cytometric staining of C57Bl/6 mouse peripheral granulocytes with Rat anti Mouse Ly-6B.2 conjugated to RPE (MCA771PE)

Flow cytometric staining of mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 (MCA771GA)

Flow cytometric staining of mouse peripheral blood granulocytes with Low endotoxin Rat anti Mouse Ly-6B.2 (MCA771EL)

Flow cytometric staining of mouse peripheral blood granulocytes with RPE conjugated Rat anti Mouse Ly-6B.2 (MCA771PEB)

Immunoperoxidase staining of C57BL/6 mouse spleen cryosection with Rat anti Mouse Ly&-6B.2 (MCA771G)

Flow cytometric staining of New Zealand Black mouse peripheral blood granulocytes with FITC conjugated Rat anti Mouse Ly-6B.2 (MCA771FA)

Immunofluorescence staining of mouse Peyer's patch with Rat anti Mouse Ly-6B.2 conjugated to FITC (MCA771F), green; counterstained for macrophages (F4/80), red and nuclei, blue

Immunoperoxidase staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 followed by Goat anti Rat IgG (STAR72) as a detection reagent. Low power

Immunoperoxidase staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 followed by Goat anti Rat IgG (STAR72) as a detection reagent. Medium power

Immunoperoxidase staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 followed by Goat anti Rat IgG (STAR72) as a detection reagent. High power

immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

Published customer image:
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunofluorescence.
Image caption:
Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1β antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1β, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 μm in length. These micrographs were taken of dermal tissue under 630× magnification.

From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.

Published customer image:
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by flow cytometry.
Image caption:
Characterization of high dose zymosan peritonitis. A) Representative flow-cytometric density plots showing the recruitment of inflammatory cells and the clearance of fluorescent (FITC)-labelled zymosan particles. The examples shown are taken from 18 hours after the administration of zymosan. Cells were identified as described in the methods. The panel on the left shows the gating of Ly-6B+ cells, which includes (right) Ly-6Ghigh neutrophils and Ly-6G- monocytes and MØ. Both neutrophils and monocytes/MØ exhibit association with FITC-labeled zymosan at this time point (right panel). B) Graphical representation of the number of neutrophils (left) and all types of monocyte (Mo) and MØ combined (right) in the peritoneal cavity before and after acute zymosan peritonitis. n = 3 129S6/SvEv mice per group and is representative of 2 independent experiments. Data is shown as mean±SEM and cells were isolated from naïve animals (0 hours), and challenged mice 4, 18, 72 and 168 hours after induction of peritonitis. C) Graphical representation of the percentage of neutrophils and Mo/MØ present that are associated with zymosan, scored by flow-cytometry as indicated in (A) above. D) Photomicrograph, which is representative of neutrophils associated with zymosan in a 4 hour inflammatory infiltrate. Those neutrophils that are associated tend to have multiple particles, but only represent a minority of the total neutrophils present at this time (see (C) above).

From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.

Published customer image:
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.
Image caption:
Peri-incisional infiltration of neutrophils, immunohistochemical appearance. Hind paw skin from incised mice was processed for the identification of neutrophils. Panel A displays an H&E stained section of plantar hind paw skin demonstrating a predominantly dermal cellular infiltrate 2 hours after incision. The dermal and epidermal layers are labeled. Panel B displays the appearance of non-incised hind paw skin stained with a neutrophil-specific antibody. Panel C displays a micrograph taken of incised skin stained for neutrophils 2 hours after incision. Note the abundance of darkly staining infiltrating neutrophils in the dermis of this section compared to that displayed in panel B. The scale bar in panel C is 150 µm in length. All micrographs were taken using 200× magnification.

From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.