RAT ANTI MOUSE CD8 ALPHA:Pacific Blue®【科瑞奥博】

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RAT ANTI MOUSE CD8 ALPHA:Pacific Blue®

货号： MCA609PB 基本售价： 3282.0 元规格： 100 TESTS/1ml

产品信息

概述
货号	MCA609PB
克隆号	KT15
同种亚型	IgG2a
反应种属	Mouse
来源宿主	Rat
应用	F

性能
供应商	Bio-Rad Antibodies
溶解方法	Pack Size: 25 TestsReconstitute with 0.25ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
运输条件
存放说明	Pack Size: 0.25 mg, 25 µgStore at +4^oC for one month or at -20^oC for longer. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mg, 25 µgStore at +4^oC for one month or at -20^oC for longer. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mg, 25 µgStore at +4^oC for one month or at -20^oC for longer. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC. This product should be stored undiluted. DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8α antibody, clone KT15 (MCA609) followed by horseradish peroxidase conjugatedGoat anti Rat IgG (STAR72) s a detection reagent. Low power

strong>Published customer image:
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes in a mouse model of colorectal liver metastasis by immunohistochemistry and immunofluorescence on acetone treated liver cryosections.
Image caption:
(a) Immunohistochemistry and (b) immunofluorescence double staining: Representative image of CD4 (red)/CD8 (blue) (top) expression and Foxp3 (FITC)/CD4 (Cy3) (bottom) expression in liver metastases on day 10 (n = 7) compared to increased CD4 (red)/CD8 (blue) expression and Foxp3 (FITC)/CD4 (Cy3) expression on day 20. Immunohistochemistry: Nova red brick red color, Vector Blue blue color. Haemalaun blue color-nuclear counterstaining. Asterisk with indication lines show metastases next to normal tissue (×100 and ×400). Immunofluorescence: FITC green fluorescein isothiocyanate, Cy3 red and DAPI 4′,6-diamidino-2-phenylindole blue - nuclear counterstaining (×250).

From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A. Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases. BMC Cancer. 2010 Mar 7;10:82.

Published customer image:
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes in a mouse model of colorectal liver metastasis by immunofluorescence on acetone treated liver cryosections.
Image caption:
Immunofluorescence: Representative images of increased CD8+/FAS (a) and CD8+/TUNEL (b) expression on tumor infiltrating lymphocytes at the margin of liver metastases on day 20. FITC green Fluorescein isothiocyanate, Cy3 red and DAPI 4′,6-Diamidino-2-phenylindoldihydrochlorid blue - nuclear countersaining. Case demonstrates area of the TUNEL staining in serial cryostat sections. To adjust a better contrast TUNEL stained sections were not counterstained with DAPI. Asterisk with indication line shows metastatic tumor cells. Magnifications ×250 (top) and ×400 (bottom).

From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A. Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases. BMC Cancer. 2010 Mar 7;10:82.

Published customer image:
Rat anti Mouse CD8α antibody, clone KT15 use for the detection of CD8 positive T cells from tumors by flow cytometry and immunofluorescence.
Image caption:
Vaccination with pFap suppresses TAM, MDSC, Treg, and enhances DC and CTL recruitment. Primary tumors were isolated from mice 25 days after orthotopic challenge and immune cells were identified in primary tumors using antibodies for the following: (A) tumor-associated macrophages (TAMs): F4/80 (red), (B) myeloid derived suppressor cells (MDSCs): CD11b (red, nuclear) and Gr-1 (green, nuclear), (C) T regulatory cells (Tregs, white arrowheads): CD4 (red, cell surface) and FOXP3 (green, nuclear), (D) dendritic cells (DC): 33D1 (red), and (E) cytotoxic T lymphocytes (CTL): CD8 (red) Scale bar for all panels=100 μm.

From: Liao D, Luo Y, Markowitz D, Xiang R, Reisfeld RA (2009) Cancer Associated Fibroblasts Promote Tumor Growth and Metastasis by Modulating the Tumor Immune Microenvironment in a 4T1 Murine Breast Cancer Model. PLoS ONE 4(11): e7965.

Published customer image:
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
sIM-1.6.α induces enduring CD4 and CD8 T cells memory response. Mice were immunized and aerosol challenged as mentioned in Figure 1. Lymphocytes were stimulated in vitro with PPD (50 &mu:g/ml) for 48 h and later stained with fluorochrome conjugated antibodies and analyzed by flowcytometry. A, flow contours depict percentage of gated CD4 T cells expressing IL-7R and CD44 (upper panel); CD62L on CD4 T cells (lower panel). C, gated CD8 T cells expressing IL-7R and CD44 (upper panel) and CD62L on CD8 T cells (lower panel). Bar diagrams show total number of CD4 (B) and CD8 (D) T cells expressing CD44hi, CD62Lhi and CD62Llo. Data shown are mean ± SEM of two independent experiments with n = 3 animals per group. "*" and "**" indicate p<0.05 and p<0.01 respectively.

From: Singh V, Jain S, Gowthaman U, Parihar P, Gupta P, et al. (2011) Co-Administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis Infected Macrophages Vaccine Induces Better Protective T Cell Memory than BCG. PLoS ONE 6(1): e16097.

Published customer image:
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Vaccination with xIM-1.6.α induces CD4 and CD8 T cells memory response. Mice were immunized and aerosol challenged as mentioned in Figure 1. Pooled lymphocytes from spleen and lymphnodes of immunized animals were stimulated in vitro with PPD (50 μg/ml) for 48 h and later stained with fluorochrome tagged antibodies and analyzed by flowcytometry. A, flow contours depict percentage of CD4 T cells expressing IL-7R and CD44 (upper panel); CD62L expression on CD4 T cells (lower panel). C, CD8 T cells expressing IL-7R and CD44 (upper panel); CD62L on CD8 T cells (lower panel). Bar diagrams show total number of CD4 (B) and CD8 (D) T cells expressing CD44hi, CD62Lhi and CD62Llo. Data shown are mean ± SEM of two independent experiments with n = 3 animals per group. "*" indicates p<0.05.

From: Singh V, Jain S, Gowthaman U, Parihar P, Gupta P, et al. (2011) Co-Administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis Infected Macrophages Vaccine Induces Better Protective T Cell Memory than BCG. PLoS ONE 6(1): e16097.

Published customer image:
Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide immunization induces low avidity functionally impaired CTL. Splenocytes from mice immunized with peptide or DNA were analyzed at day 20 for A, Presence of antigen specific CTL by pentamer and CD8 staining. B, Frequency of epitope specific responses in IFNγ elispot assay C, Avidity and frequency of epitope specific responses by measuring responses to increasing peptide concentration in IFNγ elispot assay, D, Analysis of the avidity by normalization of responses. E, The ability of CTL lines to lyse tumor cells in chromium release assay. Data is representative of at least three independent experiments.

From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.

Published customer image:
Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide boost leads to loss of high avidity responses in vivo. A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFNγ elispot assay, D) normalization of responses to increasing peptide concentration in IFNγ elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments.

From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.

Figure A. FITC conjugated rat anti mouse CD4 (MCA2691F) and Alexa647® conjugated Rat IgG2b isotype control (MCA1212A647). Figure B. FITC conjugated rat anti mouse CD4 (MCA2691F) and Alexa647® conjugated rat anti mouse CD8 alpha (MCA609A647). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).