RAT ANTI MOUSE CD68【科瑞奥博】

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RAT ANTI MOUSE CD68

货号： MCA1957 基本售价： 3214.0 元规格： 0.25 mg

产品信息

概述
货号	MCA1957
克隆号	FA-11
同种亚型	IgG2a
反应种属	Mouse
来源宿主	Rat
应用	C, F, IF, IP, P, WB*

性能
供应商	Bio-Rad Antibodies
溶解方法	Pack Size: 25 TestsReconstitute in 0.25 ml disilled waterPack Size: 100 TestsReconstitute with 1.0 ml distilled water
运输条件
存放说明	Store at -20^oC only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

声明
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参考图片

Flow cytometric staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68 (MCA1957) following permeablisation with Leucoperm (BUF09), visualised with Mouse adsorbed FITC conjugated F(ab')2 Goat anti Rat IgG: (STAR69)

Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 (MCA1957GA) with Goat anti Rat IgG:HRP (STAR72) as a detection antibody

Immunoperoxidase staining of mouse spleen cryosection with Rat anti Mouse CD68 (MCA1957) followed by Goat anti Rat IgG:HRP (305005) showing staining of macrophages in the red pulp

Frozen mouse lymph node stained with rat anti-mouse CD68 (MCA1957) at a 1/100 dilution followed by goat anti-rat IgG:HRP (STAR72) at a 1/50 dilution.

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MCA1957) followed by Goat anti Rat IgG antibody (STAR72). High power

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 (MCA1957), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with CD68 (MCA1957A488) at a 1/5 dilution and CD88 (MCA2456A647) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by mouse Seroblock (BUF041B). Permeabilisation was with Leucoperm (BUF09)

Published customer image:
Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.
Image caption:
CB2 receptors are expressed in microglial cells and do not accumulate in Aβ plaques of APPswe/PS1ΔE9 mice. A representative confocal image of staining for CB2 receptors (green, H60 antibody) in the cortex of 12 mo-old transgenic mice. (B) An overlap of red (CD68) and blue (DAPI) channels for the image shown in A. Note a characteristic gathering of activated microglia around an amyloid plaque (marked by an asterisk). (C) An overlap of channels shown in A-B. Note that areas with high CB2 intensities overlap with CD68-positive areas. White rectangle shows an example of areas used for quantifications presented in E-F. Scale bar is 15 μm. (D) Quantification of CB2 densities (integrated intensities/area) in CD68-positive and –negative areas. 26 areas like that shown in A-C were used for the quantification (n = 2 transgenic mice). Asterisk indicates a significant difference between CD68+ and CD68- areas (one-way ANOVA, p<0.0001). (E) A scatterplot of CB2 and DAPI intensities as a function of distance from the center of an Aβ plaque with radius ~10 µm. Note low CB2 signal in the core of the plaque. CB2 and DAPI intensities were normalized (%) to a maximum signal on each channel. An example of an area used for calculations is shown by a white rectangle in C. (F) Quantification of CB2 signal at different distances from a plaque center. 4–6 slices of z stacks from five plaques (range of radiuses 7–15 μm) were used in one-way ANOVA. Asterisks indicate a significant increase (p<0.0001, post-hoc test) in CB2 intensities as compared to the core of plaques (radius = 7 μm).

From: Savonenko AV, Melnikova T, Wang Y, Ravert H, Gao Y, Koppel J, et al. (2015) Cannabinoid CB2 Receptors in a Mouse Model of Aβ Amyloidosis: Immunohistochemical Analysis and Suitability as a PET Biomarker of Neuroinflammation.
PLoS ONE 10(6): e0129618.

Published customer image:
Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.
Image caption:
Localization of CB2 receptors in microglial cells and engulfment synapses. (A) A confocal image of CB2 (green) and CD68 staining (red) centered at the core of an Aβ plaque (marked by an asterisk). Note a region of high intensity for CB2 and CD68 staining around the plaque implying that CB2 receptors are localized in microglial processes surrounding the plaque. Scale 15 μm. (B) A magnification of a microglia cell body (red) and its process (red) forming an engulfment synapse on a dense core amyloid plaque (marked by an asterisk, DAPI). Note CB2 receptor staining along the edge of CD68-positive staining and at the engulfment synapse. White boxes indicate areas used for quantifications in C-D. (C-D) Quantification of CB2, CD68, and DAPI signals from the image of a microglia cell body (C) and engulfment synapse (D). The quantification was done using a Plot profile analysis tool (Fiji). Signals were averaged along short axes of boxes shown in B and normalized to a max value (100%) for each channel. Units of X axis are pixels, scale: 11.1 pixels/ μm. (E) 3D reconstruction of the engulfment synapse shown in B, D. A z stack of 0.31 μm slices (n = 29) was processed by using a background subtraction function and normalization for each of the channels. Surfaces for the plaque (DAPI), microglia process (CD68), and CB2 signal were created by arbitrary thresholding at an upper third of intensity distributions. Note high intensities of CB2 signals are located between CD68 and DAPI surfaces. Insert shows orientation of a 3D window as related to a position of the brain slide.

From: Savonenko AV, Melnikova T, Wang Y, Ravert H, Gao Y, Koppel J, et al. (2015) Cannabinoid CB2 Receptors in a Mouse Model of Aβ Amyloidosis: Immunohistochemical Analysis and Suitability as a PET Biomarker of Neuroinflammation.
PLoS ONE 10(6): e0129618.

Published customer image:
Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.
Image caption:
Comparison of CB2 immunoreactivity in neurons, activated microglia and astrocytes. (A) Representative confocal images from the cortex of 12 mo-old non-transgenic (NTG) and APPswe/PS1ΔE9 transgenic (AD) mice stained with a CB2 receptor antibody (H60sc; left columns; green) and markers for neurons (NeuN, far red), activated microglia (CD68, red), and astrocytes (GFAP, far red). Brain slides were counterstained with DAPI shown with a grey pseudo color. Note substantial micro- and astro-gliosis in the cortex of the AD mouse brain. In the NTG mice, CD68+ and/or GFAP+ areas were rare (indicated in the upper right panel by an arrowhead and arrow, respectively). (B) Quantification of densities (+-SEM) for CB2 receptor immunoreactivity (integrated intensities/area) in areas positive for NeuN, CD68, and GFAP markers. Densities were averaged over 22 (AD) and 14 (NTG) images of the cortex as shown in A (n = 2 mice per genotype). Single and double asterisks indicate a significant difference between NTG and AD groups as a result of LSD post-hoc test with p levels <0.01 and 0.0001, respectively. Arcs indicate non-significant (NS) differences. Single and double pound signs (p levels <0.05 and 0.001) indicate markers that correspond to the highest CB2 density in the NTG (blue sign) or AD (red sign) groups (LSD post-hoc test). Solid black line at the level of 4,930 shows average densities for the background. (C) An example of NeuN (blue), CD68 (red), and GFAP (green) masks from the AD image shown in A. NeuN masks were drawn by hand as shown in Fig 2C; masks for CD68 and GFAP were created by a threshold function. Black area represents background. Scale is 15 μm.

From: Savonenko AV, Melnikova T, Wang Y, Ravert H, Gao Y, Koppel J, et al. (2015) Cannabinoid CB2 Receptors in a Mouse Model of Aβ Amyloidosis: Immunohistochemical Analysis and Suitability as a PET Biomarker of Neuroinflammation.
PLoS ONE 10(6): e0129618.

Published customer image:
Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse brain by immunohistochemistry on cryostat sections.
Image caption:
Primed innate immune response in the brain following a systemic bacterial infection. Phenotypic changes after intracerebral injection of 100 pg of LPS in naïve mice (A, B, E, F, I, J, M, N) or mice pretreated with SL3261 (C, D, G, H, K, L, O, P). Representative images of immune marker expression (CD11c (A-D), CD68 (E-H), MHCII (I-L) in the injected hemisphere (B, D, F, H, J, L, N, P) or contralateral site are shown. Panels M-P show a double immunofluorescent stain for MHCII (green) and laminin (red). n=5 animals per group, black scale bar=50μm, white scale bar=75μm.

From: Püntener U, Booth SG, Perry VH, Teeling JL. Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia.
J Neuroinflammation. 2012 Jun 27;9:146.

Published customer image:
Mouse anti CD68 antibody, clone FA-11 used for the detection of macrophages in mouse by flow cytometry.
Image caption:
F4/80 and either CD11b or CD68 expression of whole liver MNCs. Liver MNCs were obtained from CD, HFD, HCD and HFCD mice and expression of F4/80 either with CD11b or CD68 were examined. The numbers were the means±SE from four mice in each group. F4/80 positive gate was determined by using the isotype control Ab.

From: Nakashima H, Ogawa Y, Shono S, Kinoshita M, Nakashima M, et al. (2013) Activation of CD11b+ Kupffer Cells/Macrophages as a Common Cause for Exacerbation of TNF/Fas-Ligand-Dependent Hepatitis in Hypercholesterolemic Mice. PLoS ONE 8(1): e49339.