| 货号 | MCA2189F |
| 克隆号 | 2G5 |
| 同种亚型 | IgG2b |
| 反应种属 | Mouse |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Mouse anti Mouse MHC Class I antibody, clone 2G5 (MCA2189) used for the evaluation of MHC class I expression on guinea pig lung infiltrates in a mycobacterial disease model by flow cytometry. Image caption: Evaluation of neutrophil infiltration and MHC expressions in the infected lung. The expression of MHC I, MHC II and percentage of neutrophils were monitored at different time points during the course of therapy. The bar diagram shows the mean percentage of MHC expression on gated population or neutrophil content in the lungs of drug or drug plus MIP group. Data represents the mean value of four animals in each group. * p<0.05. Entire lymphocyte negative region was gated based on forward versus side scatter and MHC expression or neutrophil infiltration was analyzed on the gated region. The representative plots show the MHC expression or neutrophil infiltration in different experimental groups along with the suitable negative and positive controls. Percentage value has been mentioned in the assigned region in each plot. From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, Katoch V, et al. (2012) Efficacy of Mycobacterium indicus pranii Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung. PLoS ONE 7(7): e39215. | |
Published customer image: FITC conjugated Mouse anti Mouse MHC Class I antibody, clone 2G5 (MCA2189F) used for the evaluation of MHC class I expression on murine retinal epithelial cells by flow cytometry. Image caption: Functional characteristics of mouse iPS-RPE. (a and b) Two types of pRPE were compared with mouse iPS-RPE. Phase-contrast images of 2 week-cultured pRPE obtained from adult mice (pRPE (2w), a) and 2 day-cultured pRPE obtained from PN day10-mice (pRPE (2d), b) are shown. (c) The expression of RPE functional marker mRNAs in fresh PN day10-RPE, DD29 iPS-RPE, pRPE (2d), and pRPE (2w) is shown. The data for fresh RPE and iPS-RPE are the same as those shown in Fig 4. (d and e) Rod outer segment phagocytosis assay. Phase-contrast image and fluorescent image of iPS-RPE that was co-cultured with or without FITC-ROS and washed by medium are shown (d). Both iPS-RPE and pRPE (2w) phagocytosed FITC-ROS, and the percentage of FITC-positive cells in iPS-RPE was significantly higher than that in pRPE (2w). Tukey-Kramer test. n = 3. (f) Immune surface antigen expression evaluated by flow cytometry. The red line histograms represent isotype control. Numbers in the histogram indicate the percentage of positive cells. (g and h) Percentage of Ki-67 and CD4 double-positive T cells (g) and that of Ki-67 and CD8 double-positive T cells (h) after stimulation by anti-CD3 and anti-CD28 antibody. iPS-RPE significantly suppressed the proliferation of CD4-positive T cells and CD8-positive T cells. Tukey-Kramer test. n = 3. Scale bars: 200 μm (a, b) and 100 μm (d). **: p<0.01, *: p<0.05. From: Iwasaki Y, Sugita S, Mandai M, Yonemura S, Onishi A, Ito S-i, et al. (2016) Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool. PLoS ONE 11(7): e0158282. |
