| 货号 | MCA519PET |
| 克隆号 | MOMA-2 |
| 同种亚型 | IgG2b |
| 反应种属 | Mouse |
| 来源宿主 | Rat |
| 应用 | F* |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute with 0.25 ml distilled water |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. Following reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of mouse peritoneal macrophages with FITC conjugated Rat anti Mouse Macrophages/Monocytes (MCA519FA) following permeabilisation with Leucoperm (BUF09) | |
Staining of mouse peritoneal macrophages with RPE conjugated Rat anti Mouse Macrophages/Monocytes (MCA519PE) following permeabilisation with Leucoperm (BUF09) | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in atherosclerotic aotic lesions from control and ApoE knockout mice. Image caption: MOMA-2 staining of ApoE-/- (SKO) and ApoE-/-, P-selectin-/- (DKO) mice fed a chow diet. Macrophages, stained by MOMA-2 monoclonal antibody, are shown binding the endothelial layer and penetrating the intimal lesions in SKO (a, c) and DKO (b, d) animals following, respectively, 15- (a, b) and 20- (c, d) week chow diet. Black arrows show immunostained macrophages. Original magnification: x250. From: Marie-Claude Bourdillon, Jacques Randon, Lydie Barek, et al., “Reduced Atherosclerotic Lesion Size in -Selectin Deficient Apolipoprotein -Knockout Mice Fed a Chow but Not a Fat Diet ,” Journal of Biomedicine and Biotechnology, vol. 2006, Article ID 49193, 8 pages, 2006. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in adipose tissue of mice using immunohistochemistry. Image caption: High number of preadipocyte/macrophage-like cells in the adipose tissue of Nscl-2 (-/-) and Nscl-2 (-/-)×ob/ob compound mutant mice. (A) Number of preadipocyte/macrophage-like cells per 250 adipocytes (F = 250.71; dF = 3; p<10-4; 4 mice per group). (B) Number of MOMA-2 positive but F4/80 negative cells per 250 adipocytes. The difference between MOMA-2 and F4/80 positive cells corresponds to adipocyte precursors (p = 0.038). (C) Richardson-stain of a section of epididymal adipose tissue from 6 months old male double mutant mouse. Arrow indicates groups of preadipocyte/macrophage-like cells between fat cells (scale bar: 25 µm). (D) MOMA-2 staining of paraffin embedded epididymal adipose tissue of a Nscl-2 (-/-) mouse (scale bar: 100 µm). The inlet shows a typical staining of MOMA-2 (scale bar: 15 µm). From: Ruschke K, Ebelt H, Klöting N, Boettger T, Raum K, Blüher M, et al. (2009) Defective Peripheral Nerve Development Is Linked to Abnormal Architecture and Metabolic Activity of Adipose Tissue in Nscl-2 Mutant Mice. PLoS ONE 4(5): e5516. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in mouse liver by immunofluorescence on formalin fixed tissue sections. Image caption: S. Typhimurium Reside in Macrophages That Appear to Have Multiple Nuclei Confocal fluorescence microscopy of 50-μm-thick liver sections from a 1-wk-infected Slc11a1 (Nramp1) wild-type mouse. S. Typhimurium (O-antigen, arrows) are red, macrophages (F4–80 and MOMA-2) are blue, DNA (DAPI) is gray, and phalloidin is green. (A) Low power image, scale bar is 40 μm. (B–H) Montage of 4-μm optical sections through the boxed region of (A). Scale bar is 20 μm. (I and J) Enlarged images showing actin rings (arrowheads) around nuclei within the multinucleate macrophage. The endogenous macrophage nucleus is visible in (I) and is labeled with an N. From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection. PLoS Pathog 3(12): e193. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in mouse liver by immunofluorescence on formalin fixed tissue sections. Image caption: S. Typhimurium-Infected Macrophages Containing Phagocytosed Neutrophils and T Cells Confocal fluorescence microscopy of 50-μm-thick liver sections from 1-wk-infected Slc11a1 wild-type mice. (A–C) S. Typhimurium (O-antigen, arrows) are red, macrophages (F4–80 and MOMA-2) are blue, DNA (DAPI) is gray, phalloidin is green, and neutrophils (Gr-1/Ly-6G/RB6-8C5) are pink (arrowheads). (A) Collapsed image from a 40-μm Z-stack. Scale bar is 20 µm. (B and C) Sections from (A) that are 4 μm apart. (D–G) T cells within multinucleate macrophages. Macrophages (F4–80 and MOMA-2) are blue (D, G, and H), T cells (CD3zeta) are red (D, G, arrowheads), DAPI is gray (E, G), actin-bound phalloidin is green (F, G). (G) Is a composite of (D, E, and F). Scale bars are 16 μm. (H) An image from a different mouse stained and labeled as described for (D–G). Scale bar is 8 µm. From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection. PLoS Pathog 3(12): e193. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of bone marrow derived macrophages in mouse by immunofluorescence. Image caption: ctivated Bone Marrow–Derived Macrophages (BMDMs) Phagocytose Live Host Cells Confocal fluorescence microscopy. BMDMs are blue (F4–80 and MOMA-2), human T cells (Jurkats) are green (CMFDA-stained), and DNA is gray (DAPI). (A–C) 30 min after the addition of Jurkats to BMDMs. (A) Unactivated BMDMs show little association with Jurkat cells, many of which were washed away. Scale bar is 40 μm (B and C). Activated macrophages phagocytose Jurkat cells; arrow denotes engulfed cell, arrowheads show partially engulfed cells. Scale bars are 40 μm (B) and 8 μm (C). (D–F) 42 h after mock-infection (D), or S. Typhimurium-infection (O-antigen, red, arrows, E and F). Scale bars are 20 µm (D), 40 µm (E), and 16 µm (F).. From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection. PLoS Pathog 3(12): e193. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in aortic atherosclerotic lesions by immunohistochemistry on cryosections. Image caption: Representative sections with immunohistochemical analysis. Apo E-knockout mice were fed a Western-type diet and treated with PBS (control group) (n = 10) or 1 mg/kg/day 15d-PGJ2 (15d-PGJ2 group) (n = 10) for 2 mo. Representative cross-sections of the aortic sinus were stained with MOMA-2 (A), which detected macrophages, and MCP-1 Abs (B), MIF Abs (C), TNF-a Abs (D), MMP-9 Abs (E), PPARγ Abs (F), and counterstained with hematoxylin. Right sections are control group and left ones are 15d-PGJ2 group in each figure. Black arrows indicate the positive lesions. From: Seno T, Hamaguchi M, Ashihara E, Kohno M, Ishino H, Yamamoto A, et al. (2011) 15-Deoxy-Δ12,14 Prostaglandin J2 Reduces the Formation of Atherosclerotic Lesions in Apolipoprotein E Knockout Mice. PLoS ONE 6(10): e25541. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used to immunostain RAW 264.7 cells using immunofluorescence. Image caption: Immunofluorescence and western blot analysis of MMP-2 and MMP-9 expression in vitro. Panel A shows the immunofluorescence images of MMP-2 in macrophages receiving doxycycline, simvastatin or no treatment. Panel B shows the immunofluorescence images of MMP-9 in macrophages receiving doxycycline, simvastatin or no treatment. Panel C shows western blot analysis of MMP-2 and MMP-9 expression. Panel D and E are the quantitative analysis of Panel C. Blue color represents DAPI staining, green color MOMA-2 staining and red color MMP-2 or MMP-9 staining. Bars = 20 μm. Group D: doxycycline-treated group; Group S: simvastatin-treated group; Control: control group. **P<0.01, vs. Control group. From: Dong M, Zhong L, Chen WQ, Ji XP, Zhang M, Zhao YX, et al. (2012) Doxycycline Stabilizes Vulnerable Plaque via Inhibiting Matrix Metalloproteinases and Attenuating Inflammation in Rabbits. PLoS ONE 7(6): e39695. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the demonstation of macrophages in aortic atherosclerotic lesions by immunohistochemistry on cryosections. Image caption: p210 immunization confers protection against atherosclerosis. Representative pictures of aortic en-face lipid staining from each group shown (A; left panel). Immunization with native p210 resulted in a significant reduction in aortic atherosclerosis when compared to PBS and cBSA/Alum group (A; right panel; n = 9–10 each group). P210 immunization significantly reduced macrophage infiltration (B) assessed by MOMA-2 stain (n = 9–10 each group) and DC presence (C) assessed by CD11c (n = 7–12 each group) stain in aortic sinus plaques. Positive stain indicated by reddish-brown color. Data were analyzed by ANOVA followed by Newman-Keuls multiple group comparison, *P<0.05 vs. PBS and cBSA/alum. From: Chyu K-Y, Zhao X, Dimayuga PC, Zhou J, Li X, Yano J, et al. (2012) CD8+ T Cells Mediate the Athero-Protective Effect of Immunization with an ApoB-100 Peptide. PLoS ONE 7(2): e30780. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in aortic atherosclerotic lesions by immunohistochemistry on formalin fixed cryosections. Image caption: PBA treatment influenced the stability of plaque. Representative photomicrographs and the quantitative analysis of smooth muscle cell (SMC), CD3, macrophage (Mφ), and TUNEL in lesion of aortic root of ApoE-/- mice. The aortic root sections were stained with rabbit polyclonal to α- smooth muscle actin, rat anti-mouse macrophage Moma-2 and rat anti mouse CD3. The content of macrophage, smooth muscle cell, CD3 T cell in lesion was analyzed respectively by immunohistochemistry. For detection of cell apoptosis, sections were incubated with anti-TUNEL antibody and the content of apoptotic cells was analyzed by immunofluorescence. (n = 10 per group), *p<0.05, **p<0.01, ***p<0.001. From: Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, et al. (2014) The Modulation of Endoplasmic Reticulum Stress by Chemical Chaperone Upregulates Immune Negative Cytokine IL-35 in Apolipoprotein E-Deficient Mice. PLoS ONE 9(1): e87787. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used to evaluate macrophage influx to carotid artery atherosclerotic lesions by immunohistochemistry on formalin fixed cryosections. Image caption: Carotid plaque compositions in three groups of mice. A, Representative Sirius-red staining of collagen, Oil-red O staining of lipids, MOMA-2 immunostaining of macrophages and α-SMC-actin immunostaining in three groups of mice; (B) Quantification of staining results in three groups of mice. * P<0.05, ** P<0.01, ***P<0.001 vs. Ad-EGFP group. From: Liu XL, Zhang PF, Ding SF, Wang Y, Zhang M, Zhao YX, et al. (2012) Local Gene Silencing of Monocyte Chemoattractant Protein-1 Prevents Vulnerable Plaque Disruption in Apolipoprotein E-Knockout Mice. PLoS ONE 7(3): e33497. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in atherosclerotic plaques for the aortic sinus by immunohistochemistry on cryosections. Image caption: Effects of diet change on atherosclerotic plaques in aortic sinus from apoE-/- mice. (A) Representative photograph of Oil-Red-O and macrophage (MOMA-2) staining of atherosclerotic plaques in the aortic sinus of atherogenic diet (AD) and normal diet (ND) mice. Bar = 0.1 mm. (B) Representative photograph of CD3+ T cell staining of atherosclerotic plaques in the aortic sinus of atherogenic diet (AD) and normal diet (ND) mice. Bar = 0.1 mm. (C) Histomorphometric measurements of each staining are shown in bar graphs. N = 10 in each group for plaque size, lipid content and macrophage content. N = 5 in each group for CD3+ T cell content. From: Chyu K-Y, Lio WM, Dimayuga PC, Zhou J, Zhao X, Yano J, et al. (2014) Cholesterol Lowering Modulates T Cell Function In VivoandIn Vitro.PLoS ONE 9(3): e92095. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in atherosclerotic plaques by immunohistochemistry on cryosections. Image caption: Lesion complexity does not differ with CCL3 bone marrow genotype. LDLR-/- mice were transplanted with C57BL/6 or CCL3-/- bone marrow. At four weeks post-BMT, mice were placed on WD for 12 weeks. ORO (4×), Trichrome, MOMA-2, and CD4 stained lesion (10×) are in columns as indicated on figure. Gender and donor marrow are indicated on the left of the figure. Sections were chosen from images representing mice with lesion areas close to the mean of their respective groups. From: Kennedy A, Gruen ML, Gutierrez DA, Surmi BK, Orr JS, Webb CD, et al. (2012) Impact of Macrophage Inflammatory Protein-1α Deficiency on Atherosclerotic Lesion Formation, Hepatic Steatosis, and Adipose Tissue Expansion. PLoS ONE 7(2): e31508. | |
Published customer image: Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in aortic sinus lesions using immunohistochemistry on formalin fixed cryosections. Image caption: The quantification of lesion area for macrophage abundance as calculated using Moma-positive staining. From: Wang L, Yang M, Arias A, Song L, Li F, Tian F, et al. (2015) Splenocytes Seed Bone Marrow of Myeloablated Mice: Implication for Atherosclerosis. PLoS ONE 10(6): e0125961. |
