Human/Mouse/Rat FoxP3 Allophycocyanin Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Human/Mouse/Rat FoxP3 Allophycocyanin Affinity Purified PAb (100 TESTS)

货号： IC3240A 基本售价： 5373.6 元规格： 100 Tests

产品信息

概述
货号	IC3240A
别名	AIID; AIIDMGC141961; DIETER; forkhead box P3; Forkhead Box Protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked
全称	Forkhead Box P3
反应种属	Human/Mouse/Rat
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human FoxP3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxD3 is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	50943 (Human); 20371 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A small-molecule inhibitor of macrophage migration inhibitory factor for the treatment of inflammatory disease. Authors: Kithcart AP, Cox GM, Sielecki T FASEB J., 2010;24(11):4459-66. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human FoxP3 Gln105-Lys200 Accession # Q9BZS1
生物活性	Human, Mouse, Rat
标记	Allophycocyanin
背景	Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4⁺ T cells. In human, FoxP3 is found in CD4⁺, CD8⁺ and CD4⁺CD25⁺ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.
运输条件	Blue Ice
存放说明	4℃
参考文献	Forkhead Domain Transcription Factors ICC / IHC Images: FoxP3 Multiple Sclerosis and Myelin Related Molecules Regulatory T Cells (Tregs) Transcription Factors and Regulators Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Mouse Anti-Human CD4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB3791C) or (B) Mouse Anti-Human IL‑2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB1020P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).

Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat FoxP3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3240A) and either (A) Rat Anti-Mouse CD4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat Anti-Mouse IL‑2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB2438P). Quadrant markers were set based on control antibody staining (Catalog # IC108A).