| 货号 | IC409T-100UG |
| 别名 | Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40 | 全称 | Interleukin 9 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse IL-9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human IL-9 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3578 (Human); 16198 (Mouse) |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9 Gln19-Pro144 Accession # P15247.1 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 594 |
| 背景 | Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. Mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% amino acid sequence homology. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|