| 货号 | FAB7718U-100UG |
| 别名 | CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin | 全称 | Melanoma Cell Adhesion Molecule |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse MCAM/CD146 in direct ELISAs.In direct ELISAs, less than 5%cross-reactivity with recombinant mouse (rm) MAdCAM-1 is observed, and nocross-reactivity with rmALCAM, rmNCAM, rmL1CAM, rmOCAM, rmTROP-2, recombinanthuman MCAM, or recombinant rat MCAM is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4162 (Human); 84004 (Mouse); 78967 (Rat) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse MCAM/CD146 Met1-Val563 Accession # Q8R2Y2 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 350 |
| 背景 | MCAM (Melanoma cell adhesion molecule; also CD146 and MUC18) is a 110‑120 kDa member of a small group of Ig‑superfamily molecules that includes CD239 and CD166. MCAM has also been reported at a molecular weight of approximately 150 kDa. In rodent, MCAM is reportedly expressed on neurons, endothelial cells, NK cells, neutrophils, mesenchymal stem cells and melanoma cells. MCAM appears to contribute to intercellular endothelial cell junctions, and possibly contributes to the migration of select cell types. Mature mouse MCAM is a 625 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region is 540 aa in length (aa 24‑563). It contains two V‑type Ig‑like domains (aa 24‑244) followed by three C2‑type Ig‑like domains (aa 246‑512). One cytoplasmic region splice form shows a seven aa substitution for aa 600‑648. Unlike human, rodent MCAM does not undergo a splicing event that will generate a soluble isoform. Over aa 24‑563, mouse MCAM shares 90% and 74% aa identity with rat and human MCAM, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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